细胞凋亡控基因p53、bcl-2、c-myc在大肠腺癌-癌序列中的表达  

Expression of apoptotic regulating genes bcl-2,p53 and c-myc in colorectal adenoma-adenocarcinoma sequence

作　　者：洪道显[1] 王少峰[1] 王崇文[1] 

机构地区：[1]江西医学院一附院消化产科

出　　处：《江西医药》2003年第2期100-102,共3页Jiangxi Medical Journal

摘　　要：目的 研究细胞凋亡的调控基因p53、bcl-2、c-myc在大肠粘膜癌变中的作用。方法 利用免疫组织化学SP法对30例大肠腺瘤(伴轻、中、重度异型增生各10例)和30例大肠癌的活检组织标本分别进行p53、bcl-2、c-myc蛋白表达的检测,同时采用15例正常大肠粘膜作为对照。结果 (1)正常黏膜、腺瘤和腺癌的p53蛋白表达率为6.7％、40%和73.3%。三组间有显著的差异(P均<0.05)。(2)正常粘膜、腺瘤和腺癌的bcl-2蛋白表达率分别为20％、73.3％和63.3%。腺瘤和腺癌组均高于正常对照组(P均<0.01),腺癌与腺癌组间差异不显著(P>0.05)。(3)正常粘膜、腺癌和腺癌的c-myc蛋白表达率分别为26.7%、60%和76.7%。腺瘤和腺癌组均高于正常对照组(P<均0.05),腺瘤与腺癌组间差异不显著(P>0.05)。结论 (1)bcl-2基因可能通过对细胞凋亡的抑制而在大肠的早期发生作用。(2)p53基因突变在腺瘤化为腺癌的过程中起作用。(3)c-myc基因的表达与细胞增生和肿瘤发生可能关。Objective To study the possible role of the apoptotic regulating genes (bcl-2,p53 and c-myc)in colorectal mucosa lesions to explore the mechanisms of colorectal carcinogenesis. Methods The expression of p53,bcl-2 and c-myc proteins was detemined with immunohistochemical SP method in 30 cases of colorectal adenoma with dysplasia and 30 cases of colorectal adenocarcino-ma,15 cases of normal mucosa were used as con-torls. Results (1)p53 was expressed in 6.7% of the mucosas,40% of the adenomas and 73.3% of the adenocareinomas.There were significant difference among these three groups (P<0.05). (2)bcl-2 was expressed in 20% of the normal nucosas, 73.3% of the adenomas and 63.3% of the adenocarcinomas.The rate of bcl-2 expressing cases was significantly bigher in both adenomas and adenocar-cinomas than in normal mucosas(P<0.05);There was no significant difference between adenomas and adenocarcinomas (P>0.05). (3)c-myc was expressed in 26.7% of the normal muc,osas,60% of the adenomas and 76.7% of the adenocarcinomas.The rate of c-myc-expressing cases was significantly higher in both adenomas and adenocarcinomas than in normal mucosas (P<0.05).There was no significant difference betwwen adenomas and adenocarcinomas (P>0.05). Conclusions (1)bcl-2 oncogene may play an importantrole in thiearly stage of colorectal carcinogenesis by inhibiting apoptosis. (2)The muta- tion of p53 gene participates in the transformation from colorectal adenoma to adenocarcinoma. (3)c-myc overexpression may be related with cell proliferation and carcinogenesis.

关 键 词：P53 bcl—2 c—myc 大肠腺癌 癌序列 表达 细胞凋亡 

分 类 号：R735.34[医药卫生—肿瘤]