培养人视网膜色素上皮细胞在PVR患者视网膜下液作用下c-fos和c-jun的表达  

Effects of subretinal fluid on expression of c-fos and c-jun in cultured human retinal pigment epithelium

作　　者：余建洪[1] 邢怡桥[1] 项奕[1] 叶美红[1] 喻长泰[1] 艾明[1] 杨安怀[1] 彭坤[1] 

机构地区：[1]武汉大学人民医院眼科,湖北武汉430060

出　　处：《眼科新进展》2003年第3期164-166,共3页Recent Advances in Ophthalmology

基　　金：湖北省自然科学基金资助(编号 :2 0 0 1ABB13 6)

摘　　要：目的　观察培养的人视网膜色素上皮细胞 ( human retinal pigment epithelium,h RPE)在不同程度增生性玻璃体视网膜病变 ( proliferative vitreoretinopathy,PVR)患者视网膜下液 ( subretinal fluid,SRF)作用下细胞增生与 c- fos和 c- jun的表达。方法　不同程度PVR患者 SRF作用于培养的 h RPE细胞后利用细胞计数和 MTT比色实验观察 SRF对培养 h RPE细胞的作用 ,同时采用 SP法在不同时间点作兔抗人 c- fos多抗和兔抗人 c- jun多抗的免疫组织化学染色。结果　 PVR患者 SRF作用组与对照组相比可明显促进培养h RPE细胞的增殖 ,同时实验组 h RPE细胞 c- fos和 c- jun均表达增高 ,视网膜下液作用 1h后 ,Fos蛋白抗体染色阳性 ,细胞核呈明显棕黄色染色 ;3h表达达高峰 ,持续约 12 h;视网膜下液作用 1h后 ,Jun蛋白抗体染色阳性 ,细胞核呈明显棕黄色染色 ;6 h表达达高峰 ,持续约 4 8h,对照组 h RPE细胞 (未经 SRF作用仅加入等量 PBS) Fos蛋白抗体和 Jun蛋白抗体染色均为阴性。结论　 PVR患者 SRF中含有能诱导 c- fos和 c- jun等“立早基因”转录的细胞外生长或分化因子 ;c- fos和 c- jun的表达是 SRF促进Objective To observe the expression of c fos and c jun in cultured human retinal pigment epithelium (hRPE) after treatment with subretinal fluid (SRF) from different grades of proliferative vitreoretinopathy (PVR).Methods The effect of SRF from different grades of PVR on cultured hRPE was detected by the methods of cell counting and MTT assay. SP techniques were applied for immunohistochemical staining of cultured hRPE with rabbit anti human c fos antibodies and rabbit anti human c jun antibodies.Results All SRF samples showed a stimulating effect on cultured hRPE in a certain degree compared with the control group.While all SRF samples could stimulate the expression of c fos and c jun in cultured hRPE.The positive staining was mottled in nucleus.There was significant difference between the SRF treated group and the control group.Conclusion SRF from PVR includes some growth factor that can induce the expression of c fos and c jun in cultured hRPE.We can conclude that hRPE cell proliferation is under fos and jun control.

关 键 词：人视网膜色素上皮细胞 原癌基因 免疫组织化学 视网膜下液 

分 类 号：R776.4[医药卫生—眼科]