维生素E琥珀酸酯诱导胃癌细胞凋亡  

Apoptosis induced by vitamin E succinate in gastric cancer cells

作　　者：李贵昌[1] 吴坤[1] 赵艳[1] 赵岚[1] 

机构地区：[1]哈尔滨医科大学公共卫生学学院营养与食品卫生教研室,哈尔滨150001

出　　处：《中国公共卫生》2003年第6期688-689,共2页Chinese Journal of Public Health

摘　　要：目的　探讨维生素E琥珀酸酯 (VES)诱导人胃癌SGC - 790 1细胞凋亡执行阶段的机制。方法　分别将对照组 0 1 %无水乙醇和 5 ,1 0 ,2 0 μg/mlVES加入培养液中 ,2 4h后通过荧光染色观察细胞核形态和线粒体跨膜电位(ΔΨm)改变 ,并用Western蛋白印记检测凋亡诱导因子在线粒体和细胞质中的重新分布。结果　VES可明显诱导人胃癌SGC - 790 1细胞凋亡 ,2 0 μg/mlVES可使细胞凋亡率达到 49 7% ,伴有ΔΨm 的明显降低 ,并有凋亡诱导因子自线粒体中的释放。结论　VES可能是通过使线粒体通透性改变 ,降低ΔΨm,并释放线粒体中的凋亡诱导因子 ,诱导SGC - 790Objective To investigate the molecular mechanism of apoptosis induced by vitamin E succinate (VES) in gastric cancer cell line SGC-7901.Methods Cells were treated with 0.1% anhydrous alcohol as control and VES in 5,10,20?μg/ml for 24h.Apoptosis and mitochondrial transmembrane potential(ΔΨ m) were morphologcally assessed through staining with fluorochrome.Mitochondria-nuclear translocation of apoptosis inducing factor (AIF) was detected with Western blotting.Results VES can induce apoptosis significantly in gastric cancer cell line SGC-7901.The apoptotic cells were 49.7% when treated with 20?μg/ml VES,which was associated with the decrease of ΔΨ m and the redistribution of AIF from mitochondria to cytoplasm.Conclusion VES may induce apoptosis of SGC-7901 through mitochondrial permeability transition which decreased the ΔΨ m and released AIF from mitochondia.

关 键 词：维生素E琥珀酸酯(VES) 胃癌 线粒体通透性 凋亡诱导因子 

分 类 号：R735.2[医药卫生—肿瘤]