p53蛋白和p53mRNA在牙源性囊肿和成釉细胞瘤中表达的意义  

Expression of p53 mRNA in Odontogenic Cysts and Tumors

作　　者：钟鸣[1] 王秀侠[2] 李自娟[1] 王洁[1] 

机构地区：[1]中国医科大学口腔医学院病理学教研室,辽宁沈阳110002 [2]沈阳市口腔医院

出　　处：《中国医科大学学报》2003年第3期224-225,共2页Journal of China Medical University

基　　金：辽宁省教育厅基金资助项目 ( 2 0 12 2 17)

摘　　要：目的 :探讨成釉细胞瘤 (AB)和牙源性角化囊肿 (OKC)中p5 3蛋白及p5 3mRNA表达的生物学意义。方法 :采用免疫组化S P法和原位杂交方法分别对p5 3蛋白和p5 3mRNA进行检测。结果 :p5 3蛋白在AB、OKC及正常口腔粘膜上皮细胞核中表达阳性率分别为 85 7%、4 4 0 %和 33 3% ,组间差异显著 (P <0 .0 1)。伴随AB的复发与恶变 ,p5 3蛋白阳性表达强度增加 ,核表达数目增多 ,组间差异显著 (P <0 .0 1)。角化细胞颗粒细胞p5 3蛋白表达减少或缺失 ,但却有p5 3mRNA的阳性产物。p5 3mRNA与p5 3蛋白之间存在高度负相关 (r =- 0 95 7,P <0 .0 1)。结论 :p5 3蛋白和p5 3mRNA的表达是判断成釉细胞瘤预后的指标。Objective: Our aim was to study the expression of p53 mRNA and p53 protein in amleoblastoma (AB) and odontogenic keratocyst (OKC). Methods: The p53 mRNA and protein were determined with S-P method and in situ hybridization. Results: The positive rates of p53 protein in AB, OKC, and normal oral mucosa were 85.7%, 44.0% and 33.3%. There were statistical differences among groups (P<0.01). Accompanied with the recurrence and transformation, the positive rates of p53 protein and the number of nuclei with positive expression of p53 potein were increased. The differences among groups were significant (P<0.01). In keratinocyte and granule cell, the expression of p53 protein was low or negative, but that of p53 mRNA was positive. The relationship between p53 mRNA and p53 protein was highly negative (r=-0.957, P<0.01). Conclusion: The expressions of p53 mRNA and p53 protein are important signs to evaluate the prognosis of AB.

关 键 词：成釉细胞瘤 牙源性角化囊肿 P53基因 免疫组化 原位杂交 

分 类 号：R739.82[医药卫生—肿瘤]