组织学与核磁共振检测大鼠癫痫源性早期脑损伤的跨半球扩布特征  

Propagation of brain injuries from artificial focus into the opposite hemisphere at the early stage of rat electrogenic epilepsy identified by histology and magnetic resonance image

作　　者：韩丹[1] 臧颖[1] 杨运煌[2] 刘买利[2] 王文挺[1] 邹祖玉[3] 

机构地区：[1]武汉大学医学院生理学系 [2]中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室 [3]武汉大学医学院病理学与病理生理学系,武汉430071

出　　处：《生理学报》2003年第3期296-302,共7页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNaturalScienceFoundationofHubeiprovince (No 99J137)andtheStateKeyLaboratoryofMagneticResonanceandAtomicandMolecularPhysics (No 99T15 0 9)

摘　　要：慢性强直电刺激 ( 60Hz,2s)大鼠右侧背海马 (hippocampus ,DHPC)CA1基树突区 ,1次 /d ,连续刺激 10d。分别在施加强直电刺激的第 2、4、6、8或 10d时进行核磁共振成像检测 (T2 weightedmagneticresonanceimage ,T2 WI) ,并对鼠脑进行组织学切片鉴定。结果表明 ,早期慢性癫痫源性脑损伤的病理性形态特征主要包括 :( 1)T2 WI检测侧脑室 (lateralventricle,LV)区域信号增强、组织学检测LV扩大和双侧对称性脉络膜丛病理性增生 ,后两者并非完全平行呈现。 ( 2 )组织学切片显示双侧LV扩大面积与T2 WI信号增强区域面积的脑区分布近似。与空白对照组大鼠相比 ,电刺激 2、4、6、 8和 10d后 ,T2 WI信号增强区域面积显著增大 (P =0 0 2 5 9;P =0 0 184;P =0 0 184;P =0 0 40 4;P =0 0 2 5 9)以及组织学鉴定LV面积增大 (P =0 0 2 10 ;P =0 0 1;P =0 0 10 0 ;P =0 0 15 2 )。 ( 3 )定侧分析显示 ,T2 WI信号增强以及T2 WI信号增强区域面积和组织学鉴定LV面积扩大 ,在慢性刺激 6d时均以植入电极的对侧为主 ;第 10d时均以同侧为主。The popurse of this work was to study the characteristics of rat brain abnormalities at two hemispheres at the early stage of electrogenic epilepsy. Experiments were performed on 37 male Sprague-Dawley rats. Chronically repetitive tetanization (60 Hz, 2 s, 0.4-0.6 mA) was used to stimulate the right dorsal hippocampus (DHPC) of the rat brain once a day for 2, 4, 6, 8 or 10 d, respectively. The T 2 weighted magnetic resonance image (T 2-WI) were obtained from each experimental rat at the end of the experiments. Histological sections were obtained after experimentation. The results showed that the main pathologic changes at the early stage of epilepsy included: (1) T 2-WI hyperintensification, the histological enlargement of lateral ventricle (LV) and pathological hyperplasia of ventricular choroidea plexus occurred. The pathological hyperplasia was symmetric in two hemispheres, but the LV enlargement was not. (2) Histologically enlarged LV area showed a resemblance to T 2-WI hyperintensive area. Compared with the control rats, large T 2-WI hyperintensive area (P=0.0259; P=0.0184; P=0.0184; P=0.0404; P=0.0259) and histologically enlarged LV area (P=0.0210; P=0.01; P=0.0100; P=0.0152) were present in chronically tetanized rats. (3) Dynamic characteristics of histologically enlarged LV area resembled to those of T 2-WI hyperintensity area in chronically tetanized rats at different stimulating day. Lateralization of T 2-WI hyperintensity was in accordance with that of T 2-WI abnormal area and of histologically enlarged LV. These abnormalities were severe on the contralateral side on the stimulating day 6, or on the ipsilateral side on the stimulating day 10. These results imply characteristic propagation of brain abnormalities crossing to the opposite hemisphere at the early stage of an electrogenic rat epilepsy.

关 键 词：脑 T2加权核磁共振 侧脑室扩大 癫痫 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]