HPLC法测定七宝美髯丸中四羟基二苯乙烯苷的含量  

Determination of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glycoside in Qibaomeiranwan

作　　者：吕曙华[1] 王丽娟[1] 寿国香[1] 吕归宝[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《中国新药杂志》2003年第5期357-359,共3页Chinese Journal of New Drugs

摘　　要：目的 :建立HPLC法测定七宝美髯丸中 2 ,3,5 ,4’ 四羟基二苯乙烯 2 O β D 葡萄糖苷的含量。方法 :色谱柱为Turner C18(2 5 0mm× 4 .6mm ,5 μm) ;流动相为乙腈 水 (2 5∶75 ) ;检测波长为 32 0nm。 结果 :线性范围为14 .12～ 12 7.0 8μg·mL-1,平均回收率为 99.2 0 % ,RSD为 1.4 8% (n =5 )。结论 :本方法简便 ,灵敏 ,快速 ,准确 ,可作为该制剂质量控制标准。Objective:To establish a HPLC method for determination of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glycoside in Qibaomeiranwan(a Chinese traditional drug product).Methods:In the analysis,a Turner-C 18(250mm×4.6mm,5μm) column,and a mobile phase(25% acetonitrile solution) and a ultraviolet detector at 320 nm were adopted.Results:A linear range of 14.12～127.08 μg·mL -1,an average recovery of 99.20% with RSD of 1.48%(n=5) were obtained.Conclusion:A simple,rapid,sensitive,and specific HPLC method are established for determination of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glycoside in Qibaomeiranwan.

关 键 词：2 3 5 4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 七宝美髯丸 高效液相色谱法 药物含量 测定方法 

分 类 号：R914.1[医药卫生—药物化学]