催化材料对病毒的吸附和灭活作用及对哺乳动物细胞的毒性  

Catalytic Materials Evaluated by Adsorption and Inactivation of Parainfluenza Virus and Cytotoxicity of Mammalian Cells

作　　者：刘中民[1] 张卓然[2] 许国旺[1] 杨凌[1] 马磊[1] 孙承林[1] 许磊[1] 齐越[1] 赵春霞[1] 明平文[1] 陈严[1] 郑丛龙[2] 杜逊甫[1] 韩秀文[1] 张涛[1] 黄向阳[1] 包信和[1] 刘波[1] 刘守新[1] 王爱琴[1] 曲振平[1] 缪少军[1] 胡刚[1] 刘页[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]大连医科大学附属第二医院,辽宁大连116023

出　　处：《催化学报》2003年第5期323-327,共5页

摘　　要：提出了以吸附和催化原理灭活病毒的设想 ,旨在开发出对病毒有过滤、吸附及灭活作用的高效非特异性催化材料 ,应用于各种防护设施 ,有效控制非典型肺炎 (SARS)的传播 .采用与SARS病毒相似的副流感病毒作为模拟对象 ,进行了吸附及灭活该病毒的催化材料研究 ,并考察了催化材料对哺乳动物细胞的毒性 .结果表明 ,病毒气溶胶的阻留及吸附结果与基于DNA吸附的色谱分析结果相一致 ;部分材料可以强烈地吸附病毒 ( 10 0 % ) ,甚至在强烈振荡下并洗脱至第 3次 ,病毒也不能脱附 ;一些材料不仅可以吸附病毒 ,而且强烈振荡后的洗脱液虽然表现出一定的血凝效价 ,但接种鸡胚后 ,病毒并不增殖 ,说明材料具有明显的催化病毒灭活性能 ;对细胞毒性极低的材料可以用在与人体接触的防护材料和设施中 .筛选出的性能优异的催化材料 。A novel method for inactivating viruses based on adsorption and catalysis principles with the goal of developing non specialized materials to filtrate, adsorb and catalyze viruses is proposed. These materials would be useful to more effectively control severe acute respiratory syndrome (SARS) in China. The parainfluenza virus (PIV), which is very similar to SARS coronavirus (SARS CoV), was used in the adsorption and inactivation experiments, and the cytotoxic effects of the catalytic materials to mammalian cells were also investigated. The results of retardation and adsorption experiments on aerosol type viruses are in agreement with those of DNA adsorption and capillary electrophoresis tests. Some materials could strongly adsorb the virus with 100% efficiency even after the third acute vibration in physiological saline. Some materials exhibit an interesting effect to inactivate the virus in eluates when the latter occurred positive in hemagglutination test and negative in detection of viable virus with inoculation to chicken embryo. These results suggested that the viruses were inactivated or decomposed by the catalytic effects. The cell virulence evaluation suggested that the very low toxicity of some materials is helpful for their application in protective garments. The materials with the highest performance will be tested with SARS CoV, and the results are expected soon.

关 键 词：催化材料 病毒 吸附 灭活作用 哺乳动物 细胞 非典型肺炎 毒性 

分 类 号：R511.9[医药卫生—内科学]