巴西菇多糖对肝癌细胞株凋亡的影响及其机制  

Apoptosis induction of Agaricus blazei Murill polysaccharide in human hepatocarcinoma cells

作　　者：杨大雷[1] 张星星[2] 

机构地区：[1]广东医学院组织胚胎学教研室,广东湛江524023 [2]广东医学院附属医院血液科,广东湛江524001

出　　处：《广东医学院学报》2002年第6期418-419,共2页Journal of Guangdong Medical College

摘　　要：目的 :研究巴西菇多糖诱导人肝癌细胞株 (SMMC-772 1)凋亡的作用及机制。方法 :采用电镜、DNA电泳、免疫组化法 ,对SMMC-772 1株细胞凋亡以及凋亡抑制基因 bcl-2的表达进行检测。结果 :SMMC-772 1株细胞经巴西菇多糖处理后出现核固缩、膜起泡、凋亡小体形成 ,琼脂糖凝胶电泳呈现出典型凋亡 DNA梯状带 ,细胞内 bcl-2表达较对照组低。结论 :巴西菇多糖能诱导 SMMC-772 1株细胞发生典型凋亡 ,其抑制细胞内 bcl-2的表达 ,是诱发肿瘤细胞凋亡的机制之一。Objective:To observe the apoptosis induction and its mechanism of Agaricus blazei Murill polysaccharide(ABMPS) in human hepatocarcinoma cells.Methods:Apoptosis and bcl 2 expression of human hepatocarcinoma cell line(SMMC 7721) were detected by electronic microscopy,DNA electrophoresis and immunohistochemistry,respectively.Results:After treated with ABMPS,SMMC 7721 cells exhibited karyopyknosis,membrane vesication,apoptotic body and DNA ladder formation.Compared with non treated cells,treatment with ABMPS led to a significant reduction of bc1 2 expression.Conclusion:The apoptosis inducing activity of ABMPS in SMMC 7721 cells could be due to the inhibition of bcl 2 expression.

关 键 词：巴西菇多糖 肝癌细胞株 影响 bcl—2 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]