上皮性钙粘附蛋白和β-连环素的表达与胆囊腺癌预后的关系  

A study of E-cadherin and β-catenin expression and their correlation with prognosis of adenocarcinoma of the gallbladder

作　　者：臧长海 李新燕[2] 姚宏[2] 石立群[3] 

机构地区：[1]中华风湿病学杂志社,030013 [2]太原市人民医院病理科 [3]南京军区南京总医院病理科

出　　处：《中国药物与临床》2003年第3期210-213,共4页Chinese Remedies & Clinics

摘　　要：目的　探讨上皮性钙粘附蛋白 (E cad)及 β 连环素 (β cat)在胆囊腺癌中的表达及其与分化、转移、临床分期和预后的关系。方法　免疫组织化学法 (SP法 )和原位杂交法。结果　免疫组织化学法 :60例胆囊腺癌中E cad及 β cat的阳性率分别为 40 %和 45 % ,异常表达率分别为 58 3 %和 63 3 %。E cad和 β cat的异常表达率与胆囊腺癌的分化程度有相关性 (P <0 0 5) ,在有淋巴结转移病例中E cad及 β cat的异常表达率明显高于无转移病例 ,随着临床分期由Ⅰ期到Ⅳ期 ,E cad及 β cat异常表达率明显增高 (P <0 0 5)。E cad和 β cat异常表达病例的平均生存时间和 5年生存率明显低于正常表达病例。原位杂交法 :胆囊腺癌中E cad和 β catmRNA的阳性率分别为 60 %和 40 % ,与蛋白正常表达一致率分别为 78 8%和 82 4%。结论　检测 β cat或E cad和 β cat的共同表达是指导临床治疗及估计预后有意义的指标 。Objective To investigate the protein and mRNA expression of E cadherin and β catenin in adenoc arcinoma of the gallbladder and to find their correlation with differentiation,metastasis,TNM staging and prognosis. Methods High sensitive SP immunohistochemical method and in situ hybridization were used to detect the protein and mRNA expression of E cadherin and β catenin.Results Immunohistochemistry revealed that among the 60 cases,the positive rates of E cadherin and β catenin were 40% and 45% respectively.The abnormal expression rates of these two proteins were 58 3% and 63 3% respectively.There was no significant relationship between E cadherin and β catenin staining and histological type of the tumor ( P >0 05).However,there was a statistically significant difference between well and moderately differentiated cells and poorly differentiated cells ( P <0 05).In cases with lymphatic metastasis,the abnormal expression rates of E cadherin and β catenin were significantly higher than those in nonmetastatic cases ( P <0 05).The mean survival time in cases with abnormal E cadherin and β catenin expression was significantly shorter than that in cases with the expression grading ()～(). In situ hybridization showed that in adenocarcinoma of the gallbladder,the positive rate of E cadherin and β catenin mRNA was 60% and 40% respectively.Their concordance rates with ()～() protein expression were 78 8% and 82 4%,respectively.Conclusion The concordance rate of E cadherin and β catenin mRNA and protein expression was relatively high.They can be used as markers for prognosing adenocarcinoma of gallbladder in clinical practice.

关 键 词：上皮性钙粘附蛋白 Β-连环素 表达 胆囊腺癌 预后 

分 类 号：R735.8[医药卫生—肿瘤]