中华山荷叶内生真菌的分离及其发酵产生药用成分的初步研究  

Preliminary study on the isolation of endophytic fungus from Diphylleia sinensis and its fermentation to produce active therapeutic principle

作　　者：刘仕平[1] 曾松荣[1] 郭仕平[1] 杨春艳[1] 张玲琪[1] 

机构地区：[1]云南大学生命科学学院生物系,650091

出　　处：《中国药物与临床》2003年第3期227-228,共2页Chinese Remedies & Clinics

基　　金：国家自然科学基金资助项目( 3986 0 0 0 5);云南省农业生物技术重点实验室资助项目 ( 2 0 0 2B 0 1)

摘　　要：目的　从采自云南省中甸县的中华山荷叶 (DiphylleiasinensisLi)的根状茎中分离出可产生鬼臼毒素或其类似物的内生真菌。方法　按常规无菌方法分离内生真菌 ,纯化后接种于PDA斜面备用 ,液体培养基为改良的MM培养基。用TLC和HPLC方法对发酵产物进行检测。结果　经TLC检测 ,发现 99SJ2 1的Rf值与标样的Rf值相同。经HPLC分析 ,发现 99SJ2 1的发酵产物在相同的设定条件下与标样在tR4 34min的波峰一致。经鉴定 ,99SJ2 1为纠缠青霉 (PenicilliumimplicatumBiourage)。 结论　 99SJ2Objective To isolate the fungi which could produce podophyllotoxin or its analogues from Diphylleia sinensis in Zhongdian County,Yunnan Province.Methods The endophytic fungus was isolated by means of aseptic manipulation,cultivated with PDA medium,then fermented in modified liquid MM medium,and its fermentation products were analyzed by TLC and HPLC.Results The Rf value of 99SJ21 is the same as that of the standard sample by TLC,so is the wave crest at t R4 34 min by HPLC under same given conditions.99SJ21 was identified to be Penicillium implicatum Biourage.Conclusion 99SJ21 can produce podophyllotoxin or its analogues which have anticancer activity.

关 键 词：中华山荷叶 内生真菌 分离 发酵 药用成分 

分 类 号：R284[医药卫生—中药学]