端粒酶反义寡核苷酸对恶性脑胶质瘤细胞生长的抑制作用  

THE ROLE OF TELOMERASE OLIGODEOXYNUCLEOTIDE IN THE INHIBITION OF GROWTH OF MALIGNANT GLIOMA CELLS

作　　者：王建奇[1] 张光霁[1] 韩燕华[2] 侯利军[1] 卢亦成[1] 朱诚[1] 

机构地区：[1]第二军医大学长征医院 [2]广东省中山市人民医院博士后流动站

出　　处：《解放军医学杂志》2003年第6期514-516,F004,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 3970 0 1 4 2 )

摘　　要：为观察端粒酶反义寡核苷酸 (ODN)对恶性脑胶质瘤细胞的生长抑制作用 ,采用手术切除的端粒酶活性呈阳性表达的Ⅲ、Ⅳ级脑胶质瘤标本 1 6例 ,提取细胞进行原代培养后 ,以 5 μmol/L端粒酶反义寡核苷酸与恶性胶质瘤细胞进行孵育 ,每隔 2 4h取样 ,以流式细胞仪检测PCNA、TUNEL阳性细胞百分率及细胞周期时相。结果显示 ,反义寡核苷酸对端粒酶活性于 4 8h出现抑制 ,72h完全抑制 ,72h后对胶质瘤细胞增殖出现显著抑制并对细胞凋亡有显著促进作用 ,96h后细胞多滞留于G2 /M期 ,此期凋亡细胞百分率随时间延长而增加。提示端粒酶反义寡核苷酸对端粒酶活性具有抑制作用 ,并可引起细胞周期阻滞 。To evaluate the curative effect of antisense telomerase ODN on malignant glioma cells. The activity of telomerase in 16 fresh malignant gliomas was detected positive by PCR ELISA method. After covered with lipofectin, the 5μmol/L telomerase ODN was incubated with cultured gliomas cells. PCNA, TUNEL positive rate and the phase of cell cycle were detected by FcM per 24h. The results showed: Anti sense ODN inhibited telomerase activity after being applied for 48 hours, and inhibition was complete at 72 hours, The ODN inhibited proliferation of malignant gliomas and promoted their apoptosis markedly at 72 hours. After 96 hours, most transfected glioma cells were stopped to grow at G 2/M phase, and the number of apoptosis cells increased as time went on. The results suggested that anti sense telomerase ODN could inhibit the telomerase activity, and in turn promote aptoptosis of glioma cells and inhibit the growth of the tumor.

关 键 词：神经胶质瘤 端粒 末端转移酶 寡核苷酸类 

分 类 号：R739.41[医药卫生—肿瘤]