LPS对体外枯否细胞吞噬功能的影响  

Effect of LPS on phagocytosis of rat Kupffer cells in vitro

作　　者：贠克明[1] 韩德五[1] 许瑞玲[1] 赵元昌[1] 

机构地区：[1]山西医科大学肝病研究所,山西太原030001

出　　处：《中国病理生理杂志》2003年第6期795-798,T006,共5页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨LPS对体外培养枯否细胞吞噬功能的影响和机制。方法 :胶原酶灌流消化肝脏 ,密度梯度离心分离枯否细胞。在不同剂量脂多糖作用不同时间 ,分别用聚苯乙烯乳珠吞噬实验、荧光染色法、荧光光度法和流式细胞分析检测枯否细胞的吞噬功能、微丝和微管表达及凋亡情况。结果 :小剂量短时间作用时 ,LPS显著增强体外培养枯否细胞吞噬能力 ,但随作用时间延长和剂量的增加 ,吞噬能力反而下降 ;LPS使枯否细胞微丝和微管表达增强 ,肌动蛋白含量和微管荧光灰度显著增加 ,但剂量过大表达反而减弱 ;大剂量LPS可导致枯否细胞凋亡。结论 :LPS可以增强体外培养枯否细胞的吞噬能力 ,但剂量过大、时间过长反而抑制其吞噬能力。这可能与枯否细胞微丝、微管的变化和凋亡有关。AIM: To investigate the effect of LPS on phagocytosis of Kupffer cells in vitro. METHODS: Isolated Kupffer cells were treated with LPS in vitro . The phagocytosis, microfilament, microtubules and apoptosis of Kupffer cells were determined by the beads phagocytosis test, fluorescence staining, fluorometry and flow cytometric analysis. RESULTS: LPS enhances the phagocytosis, actin content, microtubules fluorescence density of Kupffer cells in vitro , while at a large dose or for a long time, it lessened the phagocytosis increasing or phagocytosis, inhibites the microfilament and microtubules expression, and induced apoptosis. CONCLUSION: LPS enhances the phagocytosis of Kupffer cells in vitro , but in large amount, it inhibites the phagocytosis of Kupffer cells, which is probably related to LPS -induced microfilament, microtubules expression changes and apoptosis in Kupffer cells.

关 键 词：脂多糖类 吞噬作用 枯否细胞 微丝 微管 细胞凋亡 

分 类 号：R363[医药卫生—病理学]