纤维连接蛋白对内毒素血症小鼠保护作用的实验研究  

Fibronectin supports endotoxemia mice survival

作　　者：吴勇[1] 陈元仲[1] 吕联煌[1] 

机构地区：[1]福建省血液研究所福建医科大学协和医院,福建福州350001

出　　处：《中国病理生理杂志》2003年第6期799-802,共4页Chinese Journal of Pathophysiology

基　　金：福建省科技厅基金资助项目 (No .2 0 0 1Z0 2 9)

摘　　要：目的 :研究纤维连接蛋白对内毒素血症小鼠的保护作用及其意义。方法 :采用明胶亲和层析从正常人血浆纯化纤维连接蛋白 ,观察纤维连接蛋白处理的半乳糖胺敏化内毒素小鼠的生存率 ,用病理组织学、电镜观察超微结构、DNA片段化分析、RT -PCR检测小鼠肝脏损害及TNFα、IL - 1β、IL - 6mRNA表达水平。 结果 :①纤维连接蛋白处理的半乳糖胺敏化内毒素小鼠的死亡率明显低于对照组。②病理组织学表明半乳糖胺敏化内毒素小鼠肝脏发生广泛变性坏死 ,而治疗组小鼠肝脏发生轻微变性坏死。③电镜观察半乳糖胺敏化内毒素小鼠肝脏发现多数肝细胞呈凋亡性改变 ,而纤维连接蛋白治疗组小鼠多数肝细胞大小形态结构正常 ,未见凋亡细胞。④DNA片段化分析半乳糖胺敏化内毒素小鼠肝脏细胞DNA呈现凋亡特征性梯状条带。而纤维连接蛋白治疗组则无。⑤RT -PCR研究发现纤维连接蛋白治疗组小鼠肝脏TNFα、IL - 1β、IL - 6mRNA的表达水平低于对照组。结论 :纤维连接蛋白可能通过降低半乳糖胺敏化内毒素小鼠肝脏TNF -α、IL - 1β、IL - 6的过度表达而起保护作用 ,其将成为防止败血症肝功能衰竭的方法之一。AIM: To study the preventive effect of fibronectin on hepatic failure induced by endotoxin in mice.METHODS: The survival rate was observed in endotoxemia mice injected with fibronectin from human plasma. The tissue damage and expression of TNFα, IL-1β, IL-6 mRNA in hepatocyte were detected by the methods of histology, ultrastructure, DNA fragementation and RT-PCR. RESULTS: ①Fibronectin obviously reduced the mortality of endotoxemia mice sensitized by D-galactosamine(GalN). ②Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice injected with fibronectin, compared with saline control. ③Ultrastructure and DNA fragmentation showed fibronectin suppressed hepatocyte apoptosis induced by LPS. ④Fibronectin down-regulated the overexpression of TNFα, IL-1β, IL-6 mRNA on hepatocyte induced by LPS. CONCLUSION: Fibronectin supports endotoxemia mice surrival by down-regulating the expression of TNFα, IL-β, IL-6, it may be a potent therapy for endotoxemia.

关 键 词：纤维蛋白类 内毒素血症 细胞活素类 细胞凋亡 

分 类 号：R363[医药卫生—病理学]