人髂动脉粥样硬化斑端粒酶活性检测  

Telomerase expression in human iliac artery atherosclerosis plaque

作　　者：屈艺[1] 黄翔 王正荣[1] 彭晓东[3] 刘菽秋[1] 刘柏林[1] 

机构地区：[1]四川大学华西基础医学与法医学院分子生物研究室 [2]四川省医院泌尿外科 [3]四川大学华西医院临床免疫室,四川成都610041

出　　处：《中国病理生理杂志》2003年第6期745-747,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .39970 836 )

摘　　要：目的 :探讨动脉粥样硬化形成与血管平滑肌细胞端粒酶活性表达的相关性。方法 :取 2 0例肾功能衰竭患者髂动脉粥样硬化斑及 5例脑死亡病人正常髂动脉组织 ,刮去腔面内膜层及外膜结缔组织后 ,用端粒重复序列扩增法 (Telomericrepeatsequenceamplificationprotocol,TRAP)检测剩余组织端粒酶活性。结果 :5例正常髂动脉组织均为端粒酶阴性 ,2 0例动脉粥样硬化组织中 ,有 8例为阳性 ,12例呈阴性。结论 :端粒酶与血管平滑肌细胞增生及动脉粥样硬化形成可能存在一定相关性 ,其作用待进一步研究。AIM: To observe the relationship between aortic atherosclerosis plaque formation and telomerase activation in vascular smooth muscle cells (VSMC). METHODS:Iliac artery atherosclerosis plaque from 20 renal failure patients and five cases of normal iliac arteries were obtained and confirmed by histological examination. The endothelial cells and the connective tissue on surface were lightly scraped and removed. Telomerase activity of the residual tissue was detected by telomeric repeat sequence amplification protocol. RESULTS:Five cases of normal iliac arteries showed negative telomerase activity. Eight of 20 cases athorosclerosis plaque showed positive telomerase activity, the other 12 cases showed negative telomerase activity. CONCLUSION: Telomerase activation may play a certain role in VSMC proliferation and atherosclerosis formation.

关 键 词：动脉粥样硬化 端粒酶 髂动脉 肌 平滑 血管 

分 类 号：R363[医药卫生—病理学]