利用基因芯片技术快速检测结核杆菌耐药性的初步研究  

Preliminary study on detecting drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization

作　　者：张万江[1] 鲍朗[1] 王晓樱[1] 张会东[1] 谢勇恩[1] 陈玮[1] 于向华[1] 

机构地区：[1]四川大学华西医学中心感染免疫研究室,四川成都610041

出　　处：《中国病理生理杂志》2003年第6期762-764,T003,共4页Chinese Journal of Pathophysiology

基　　金：教育部高校博士点基金资助项目 (2 0 0 0 0 6 30 0 6 )

摘　　要：目的 :探讨利用基因芯片技术检测结核杆菌的耐药性。方法 :结核杆菌耐药性检测基因芯片的制备 ;临床分离株结核杆菌培养和药敏检测 ;结核杆菌基因组DNA的制备和结核杆菌耐药基因的聚和酶链反应 ;基因芯片的杂交、洗涤、检测与分析。结果 :临床分离株结核杆菌培养和药敏检测结果 :1株为药物敏感株结核杆菌 (对异烟肼、利福平、链霉素、乙胺丁醇 4种药物均敏感 ) ;4株为多耐药株结核杆菌 (对异烟肼、利福平、链霉素、乙胺丁醇 4种药物均耐药 ) ;药敏检测结果与 5位患者临床的诊断性治疗的结果完全一致 ;进一步利用基因芯片技术进行检测 ,检测结果与上述结果相符合。结论 :利用基因芯片技术检测结核杆菌的耐药性 ,准确、快速、高效。AIM:To detect the drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization.METHODS: DNA microarray for detecting drug-resistance of mycobacterium tuberculosis was prepared; Clinical isolated strains were cultivated and their drug-resistance sensitivity was detected. The genome DNA of mycobacterium tuberculosis was prepared and the drug-resistance genes of the mycobacterium tuberculosis were amplified by PCR. Then the gene chip was hybridized, washed, detected and analyzed. RESULTS: Results of cultivating mycobacterium tuberculosis and detecting the drug-resistance sensitivity: one strain was drug-sensitive; four strains were multi-drug-resistant; The detecting results of the drug-resistance was consistent with the results of diagnosis therapy of the 5 clinical patients. The detecting results of gene chip confirmed the above facts. CONCLUSION: Detecting drug-resistance mycobacterium tuberculosis by the gene chip is precise, fast and highly-efficient.

关 键 词：寡核苷酸陈列序列分析 分支杆菌 结核 药物耐受性 

分 类 号：R363[医药卫生—病理学]