转染反义VEGF_(121) cDNA恢复K562细胞β1整合素的活化功能  

Restoring β1 Integrin Activation Function in K562 Cells Transfected with Antisense VEGF_(121) cDNA

作　　者：阮国瑞[1] 刘艳荣[1] 陈珊珊[1] 于弘[1] 常艳[1] 白任奎[1] 付家瑜[1] 

机构地区：[1]北京大学人民医院,血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2003年第3期235-237,共3页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目 编号 3 9970 3 14

摘　　要：为了探索血管内皮细胞生长因子 (VEGF)在K5 6 2细胞 β1整合素活化功能中的作用 ,利用转染正义 (S)、反义 (As)VEGF12 1cDNA及空载体 (V ,pcDNA3 )的不同K5 6 2细胞株 ,通过流式细胞术检测其粘附分子VLA 4(CD4 9d/CD2 9)和VLA 5 (CD4 9e/CD2 9)表达及 β1整合素的可活化性。结果显示 :K5 6 2 /V ,K5 6 2 /S和K5 6 2 /As细胞 β1整合素VLA 4 (CD4 9d/CD2 9)与VLA 5 (CD4 9e/CD2 9)的表达率均在 92 %以上 ,但经 β1整合素活化剂 8A2抗体激活后 ,K5 6 2 /As细胞可活化表位 9EG7表达率较K5 6 2 /V细胞明显提高 (75 .6± 10 .5vs 4 1.2± 2 .1% ,P <0 .0 1)。结论 :转染反义VEGF12 1cDNA能增加K5 6 2细胞To investigate the effect of vascular endothelial growth factor (VEGF) on β1 integrin (VLA 4 and VLA 5) activation ability in K562 cells transfected with antisense VEGF121 cDNA, K562 cells were transfected with antisense (As), sense (S) and vector (V, pcDNA 3). Flow cytometry was used to evaluate the expression rate of VLA 4 (CD49d/CD29) and VLA 5 (CD49e/CD29) and β1 integrin activation ability in the transfected K562 cells. The results showed that the expression rates of VLA 4 and VLA 5 were more than 92% in the transfected K562 cells and there was no difference among the K562/V, K562/S and K562/As cells. However, β1 integrin activated 9EG7 expression rate in K562/As cells was higher than that in K562/V cells [(75.6±10.5)% vs (41.2±2.1)%, P<0.01)] after activation with β1 integrin activator 8A2. It is concluded that function of β1 integrin to be activated is restored in K562 cells transfected with antisense VEGF121 cDNA.

关 键 词：K562细胞 血管内皮生长因子 βl整合素 慢性髓性白血病细胞系 

分 类 号：R733.72[医药卫生—肿瘤]