抗原冲击致敏的树突状细胞介导的特异性体外抗慢性粒细胞白血病细胞作用  

Specific Anti-leukemic Cell Effect Mediated by Dendritic Cells Pulsed with Chronic Myelogenous Leukemia Lysate Antigen In Vitro

作　　者：主鸿鹄[1] 徐开林[1] 潘秀英[1] 刘军权[2] 陈复兴[2] 黄一虹[1] 

机构地区：[1]徐州医学院附属医院血液科,徐州221002 [2]中国人民解放军第97医院实验科,徐州221004

出　　处：《中国实验血液学杂志》2003年第3期278-281,共4页Journal of Experimental Hematology

基　　金：江苏省卫生厅基金资助项目 编号H2 0 0 15 2

摘　　要：为研究慢性粒细胞白血病 (CML)细胞冻融抗原 (CLA)致敏的树突状细胞 (DC)对特异性抗白血病的作用 ,将CML患者外周血单个核细胞来源的DC在体外用CLA致敏 ,再与CML患者的经细胞因子诱导的杀伤细胞(CIK)共同培养。应用乳酸脱氢酶释放法观察其对自身CML细胞 ,K5 6 2细胞和Raji细胞的杀伤活性 (CIK +CLA DC组 ) ,与未致敏的DC +CIK(CIK +DC组 ) ,CIK组及CIK +CLA组进行比较。结果表明 ,效靶比为 2 5∶1时细胞杀伤活性最强 ,在该效靶比下 ,4组细胞对自身CML细胞的杀伤活性分别为 (6 8 8± 14 2 ) % ,(5 2 5± 9 4 ) % ,(2 0 7± 7 5 ) %和 (2 4 2± 8 7) %。CIK +CLA DC组杀伤活性最强 ,与其余 3组比较有显著性差异 (P <0 0 1) ;CIK +DC组比CIK组杀伤活性强 (P <0 0 1) ;CIK组与CIK +CLA组比较无显著性差异 (P >0 0 5 )。CIK +CLA DC组在效靶比为 2 5∶1时对自身CML细胞 ,K5 6 2细胞和Raji细胞的杀伤活性分别为 (6 8 8± 14 2 ) % ,(14 6± 6 2 ) %和 (12 7± 10 2 ) % ,与K5 6 2细胞和Raji细胞比较 ,具有显著性差异 (P <0 0 1)。结论 :CIK对CML患者自身细胞具有一定的杀伤活性 ;CIK与CML DC共培养组对自身CML细胞杀伤活性比CIK组强 ;CIK与CLA抗原致敏CML DC共培养组具有最强的杀伤活性。To investigate the specific antileukemia effect of dendritic cells (DC) pulsed with chronic myelogenous leukemic lysate antigen (CLA), dendritic cells from patients with chronic myelogenous leukemia (CML) were pulsed by CLA, and then cocultured with cytokine induced killer (CIK) cells from CML patients (CIK+CLA DC group). The cytotoxic activity in vitro was measured by using a lactate dehydrogenase release assay, and compared with CIK+DC, CIK and CIK+CLA groups. The results showed that under an effector target ratio of 25∶1, the cytotoxic activity of CIK+CLA DC, CIK+DC, CIK and CIK+CLA groups against autologous CML cells was (68 8±14 2)%,(52 5±9 4)%,(20 6±7 5)% and (24 2±8 7)%, respectively. CIK+CLA DC group displayed a strongest cytotoxic activity. When K562 and Raji cells acted as target cells and CIK as effectors, the cytotoxic activity against autologous CML cells in CIK+CLA DC group (68 8±14 2)%was much higher than that against K562 cells (14 6±6 2)% and Raji cells (12 7±10 2)%, respectively. In conclusion, coculture of CIK cells with DC led to a significant increase in cytotoxic activity. The cytotoxicity could be further increased by DC pulse with CML cell lysate antigen, and cytotoxicity mediated by CML lysate antigen possess stronger specificity.

关 键 词：树突状细胞 慢性拉细胞白血病 冻融抗原 细胞因子诱导的杀伤细胞 抗白血病作用 

分 类 号：R733.7[医药卫生—肿瘤] R392.11[医药卫生—临床医学]