IPT基因遗传转化谷秆两用稻的研究  

A study on genetic transformation of grain-straw-dual-use-rice with IPT gene

作　　者：许明[1] 刘峰[1] 郑金贵 

机构地区：[1]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2003年第2期205-208,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技厅资助项目(2001Z012).

摘　　要：采用农杆菌介导的方法,将嵌合基因PSAG12-IPT导入谷秆两用稻201幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得46个抗性愈伤克隆,126株转基因植株.对这些植株进行PCR检测和GUS染色分析,确定其中80株为阳性植株.Chimeric gene PSAG12IPT was transformed into embryonic calli induced from grainstrawdualuserice 201 juvenile embryo by Agrobacteriummediated method. After resistance screening of hygromycin and differentiation and growth of plantlets, 46 clones of resistance calli and 126 transgenic plants were obtained. Results of PCR detection and GUS staining analysis showed that 80 of those plants were positive.

关 键 词：谷秆两用稻 IPT基因 遗传转化 农杆菌介导 自我调控系统 叶片衰老 

分 类 号：S511[农业科学—作物学]