三种DNA片段回收纯化方法的比较  

Comparison of three kinds of methods used in DNA extraction and purification

作　　者：侯义龙[1,2,3] 姜国斌[1,2] 周其兴[1,2] 张立娟[1,2] 于亚军[1,2] 

机构地区：[1]大连大学生物工程学院 [2]大连大学生命科学工作室,辽宁大连116622 [3]中国科学院应用生态研究所,辽宁大连110016

出　　处：《大连大学学报》2003年第2期63-65,共3页Journal of Dalian University

基　　金：国家自然科学基金资助项目(30170076);辽宁省博士起动基金资助项目(20021052)

摘　　要：本文采用三种方法对苹果褪绿叶斑病毒RT-PCR的特异DNA片段进行了回收纯化。结果表明:用普通琼脂糖替代低融点琼脂糖,回收纯化后产物的浓度及纯度与低融点琼脂糖法基本一致,完全可以用普通琼脂糖替代低融点琼脂糖进行DNA片段的回收纯化,从而降低成本,简化操作.玻璃奶法的回收纯度明显高于低融点琼脂糖法和普通琼脂糖法,且更快速安全.Three kinds of methods were chosen to extract and purify DNA fragments from agarose gel. The results show that the purity and concentration of DNA fragments extracted and purified by common agarose method is almost the same as that by low melting agarose method, and the low melting agarose method can be substitued by the common agarose method. The purity and concentration of DNA fragments extracted and purified by glass milk method is obviously higher than that by the other methods. Meanwhile the glass milk method has the characteristics of safety and simplification, but the cost of it is five to six times higher than that of the common agarose method.

关 键 词：DNA片段 回收 纯化 苹果褪绿叶斑病毒 RT-PCR 基因克隆 转基因 

分 类 号：Q75[生物学—分子生物学] Q523