核酶对人巨细胞病毒mRNA片段的体外切割  

Targeted Cleavage of DNA Polymerase of Human Cytomegalovirus in vitro by the Catalytic RNA Subunit of RNase P from E. coli

作　　者：陈浩军[1] 李弘剑[1] 周天鸿[1] 周乐平[1] 何华坤[1] 魏威[1] 程华胜[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程系,广州510632

出　　处：《中国生物工程杂志》2003年第5期82-85,共4页China Biotechnology

基　　金：广东省自然科学基金资助项目 ( 0 0 718;0 2 1162 )

摘　　要：引导序列GSs(GuideSequences)是能与mRNA互补 ,引导核酶RNaseP催化核心M1RNA对互补区域特异切割的小片段游离RNA。针对人巨细胞病毒HCMV(humancytomegalovirus)DNA聚合酶mRNA序列设计GS ,共价结合到大肠杆菌来源M1RNA中 ,构建成M1GS T7核酶。通过对巨细胞病毒DNA聚合酶亚克隆片段转录产物体外切割实验 。Guide sequences(GSs) are small RNA molecules that consist of a sequence complementary to a target mRNA and render the target RNA susceptible to degradation by the catalytic RNA subunit of RNase P from Escherichia coli( M1 RNA).GS was designed to the target the mRNA encoding the major DNA polymerase of human cytomegalovirus(HCMV) for degradation.Covalent attachment to the 3′ end of M1 RNA of the guide sequence to DNA polymerase mRNA(M1GS-T7) results in very efficient cleavge of the target RNA in vitro.Thus,M1GS may represent gene-targeting agents for inhibition of HCMV DNA replication and gene expression.;

关 键 词：核酶 人巨细胞病毒 mRNA片段 体外切割 DNA聚合酶 

分 类 号：Q78[生物学—分子生物学] Q55