TNBS诱发大鼠结肠炎P选择素的表达及其与TNF-α、IL-8的相关性研究  

Association between expression of P-selectin and interleukin 8 and tumor necrosis factor α in trinitrobenzene sulphonic acid induced colitis in rats

作　　者：韩红[1] 夏冰[1] 刘君炎[2] 曾宪昌[1] 郭广松[3] 张克俭[1] 龚玲玲[3] 

机构地区：[1]武汉大学中南医院内科,武汉430071 [2]武汉大学医学院免疫学教研室,武汉430000 [3]武汉大学中南医院病理科,武汉430000

出　　处：《中国免疫学杂志》2003年第6期424-428,共5页Chinese Journal of Immunology

基　　金：湖北省教育厅科研基金 ( 99G0 0 5 )资助

摘　　要：目的 :研究P 选择素在三硝基苯磺酸 (TNBS)诱发的大鼠结肠炎组织中的表达、循环血中血小板膜表面含量的变化及其与组织TNF α和血清IL 8表达的相关性 ,探讨其在结肠炎发生发展中的作用及血栓并发症形成的可能机制。方法 :用TNBS 乙醇灌肠制作大鼠结肠炎模型 ,分别在制模后 2 4小时、1周和 2周取心脏血 ,放射免疫法测定P 选择素的含量 ,ELISA夹心法检测IL 8的含量 ,免疫组化法分析P 选择素及TNFα在病变部位的表达。结果 :与正常对照组比较 ,P 选择素在结肠组织炎症的各个时间点均有表达 ,阳性表达率大于 5 0 %。血小板膜表面P 选择素在灌肠后 2 4小时未见升高 ,第 1、2周明显升高(P <0 0 1)。血清IL 8含量及TNFα表达曲线变化情况相似 ,在 2 4小时升高最为明显 ,随着病程延长而下降。P 选择素与IL 8在同期炎症中呈正相关 ,而与TNF α无线性相关。结论 :P 选择素在结肠炎症早期介导局部的炎症反应 ,晚期仍然是维持炎症、血栓形成的重要因素之一。P 选择素含量升高和表达增强与IL 8呈正相关 ,与TNF α无线性相关。Objective:To study the expression of P selectin in the colonic mucosa and on surfaces of platelets in circulation, and determine its association with IL 8 and TNF α in trinitrobenzene sulphonic acid (TNBS) induced colitis in rat, in order to investigate the effects of P selectin on inflammation and thrombosis in inflammatory bowel disease Methods:TNBS was instilled into the colon of the rats to induce colitis Animals were sacrificed at the 24th hour, the 1st and the 2nd week after production Blood and colonic tissues were obtained P selectin on the platelets were detected by radioimmunoassay IL 8 in serum was detected by ELISA method Expression of P selectin and TNF α in colonic mucosa were assessed with immunohistochemistry Results:P selectin in mucosa was increased in different time points compared with control group The positive expression was above 50% P selectin on the platelets wasn't increased at the 24th hour, but increased at the 1st and 2nd week (P<0 01) IL 8 increased at peak at the 24th hour and then decreased similar to TNF α P selectin was correlated well with IL 8, but had no liner correlation with TNF α Conclusion:P selectin was participated in early and late stages of inflammation in TNBS induced colitis and played an important role in inflammation and thrombosis It was associated with circular IL 8, but not with expression of TNF α in colonic mucosa

关 键 词：TNBS 诱发 大鼠 结肠炎 P选择素 表达 TNF-Α IL-8 

分 类 号：R574.62[医药卫生—消化系统]