猫局灶脑缺血区氢谱分析及其病理基础的研究  

Study of 1H-MR spectra and its pathologica bases in cats with focal cerebral ischemia

作　　者：张运周[1] 尹岭[1] 朱克[1] 金香兰[1] 刘买利[2] 卢广[2] 

机构地区：[1]解放军总医院神经病学研究所,北京100853 [2]中科院武汉分院物理与数学研究所,武汉430071

出　　处：《军医进修学院学报》2003年第2期87-89,共3页Academic Journal of Pla Postgraduate Medical School

基　　金："九五"军队医药卫生科研基金 ( 96Z0 5 8)

摘　　要：目的 :对一未知氢谱 (anunknownpeak ,Pu)进行研究 ,探讨猫持续性局灶脑缺血Pu的病理基础和临床应用价值。方法 :采用猫持续性局灶脑缺血模型 ,行弥散加权磁共振 (diffusionweightedimaging ,DWI)检查 ,结合立体定向图谱 ,作为缺血后不同区域氢谱定位标准 ,在缺血后不同时间进行氢谱检查 ,除观察乳酸 (lactate,Lac)、N -乙酰天冬氨酸 (N acetylaspartate ,NAA)、肌酐 (creatine,Cr)及胆碱 (choline,Cho)等变化外 ,重点研究Pu产生时间、区域 ,并在缺血后不同时间进行HE染色 ,观察Pu产生部位的相应病理改变 ,推测Pu产生的病理基础。结果 :猫局灶脑缺血后 ,缺血脑区出现乳酸增多及NAA减少。在缺血后 2d至 7d内 ,Pu在缺血受累脑区持续存在 ,病理结果证实Pu出现的脑区局部神经细胞坏死。结论 :Pu的出现与脑缺血坏死有密切的关系 ,它可能是脑组织坏死过程中产生的一种物质 ,Pu的出现提示脑组织缺血坏死。Objective:To confirm an unknown Peak(Pu) in the permanent focal cerebral ischemia of cats with 1 H MRS, study its pathological changes and conjecture its applicatio n value in future Methods:After focal cerebral ischemia, diffus ion weighted imaging(DWI) was used to identify regions of ischemia for 1 H MRS voxel localization Meanwhile, the stereotaxic atlas of the cat brain was referred 1 H MRS was used to monitor the progression of focal cerebral ischemia in 6 cats over a period of 7 days following middle cerebral artery oc clusion Lactate, NAA, choline, creatine, and Pu were obtained The time course and cerebral areas that the Pu was detected w ere studied emphatically Meanwhile, the hematoxylin and eosin staining was use d to reveal the pathologica changes, and the pathologica bases of Pu were guesse d Results:In the affected regions, lactate was elevated a lmost immediately after the onset of cerebral ischemia, and NAA began to decline within several hours of acute infaction The Pu in the δ 1 2 region was cons istently detected in the affected cerebral areas from 2 days to 7days after MCAO The time when Pu could detected the neurons had died completely in the affect ed cerebral areas Conclusion: Pu has a close relationship with cerebral ischemia necrosis It may be a intrinsic production of the necrotic t issue, can be taken as a specific marker, and has vary important clinic applicat ion value

关 键 词：猫 脑缺血 氢谱分析 病理 乳酸 N--乙酰天冬氨酸 肌酐 

分 类 号：R743.34[医药卫生—神经病学与精神病学]