血管内皮生长因子-C基因转染对Tca8113细胞转移能力影响的研究  

The Effect of Gene Transfection of Vascular Endothelial Growth Factor-C on the Metastasis Ability of Tca8113

作　　者：华成舸[1] 潘剑[1] 温玉明[1] 李龙江[1] 王昌美[1] 

机构地区：[1]四川大学华西口腔医院口腔颌面外科,610041

出　　处：《华西口腔医学杂志》2003年第3期171-173,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (编号 39970 796)

摘　　要：目的　研究血管内皮生长因子_C(VEGF_C)对鳞癌细胞淋巴道转移能力的影响 ,以探讨其在口腔黏膜鳞癌淋巴道转移机制中的作用。方法　用本课题组前期建立的VEGF_C高表达舌癌细胞株 ,以裸小鼠皮下接种构建动物肿瘤模型 ,观察VEGF_C高表达对移植瘤的生长和颈淋巴转移的影响。结果　VEGF_C高表达移植瘤实验组的颈淋巴结转移率高于对照组 ,但实验组与对照组的移植瘤原发灶生长速度没有明显差异。结论　VEGF_C对舌鳞癌的淋巴道转移有相对特异的作用 。Objective\ To study the role that vascular endothelial growth factor_C plays in the lymph node metastasis of the squamous cell carcinoma (SCC) of tongue.Methods\ An established VEGF_C transfected Tca8113 cell strain (VcTca) was heterotransplanted into BALB/C nude mice subcutaneously. The expression of VEGF_C and VEGFR_3 was detected. The property of tumorigenesis and metastasis of the transfected cell was investigated.Results\ Jugular lymphatic metastases developed in 4/6 mice of VcTca group in 6 weeks after transplantation, while no lymph node metastasis was found in mice with tumors of non_transfected Tca8113 cell (Tca) and mock transfected cell(pDTca) at the same time. There was no difference in tumor weight among VcTca, pDTca and Tca (P>0 05).Conclusion\ It is suggested that VEGF_C could promote lymph node metastatic ability of Tca8113 cell, and could be utilized as a target of new regimen in prevention and treatment for lymphatic metastasis of oral carcinomas.\;

关 键 词：血管内皮生长因子-C 基因转染 细胞株 鳞癌细胞 肿瘤淋巴结转移 肿瘤转移 

分 类 号：R739.8[医药卫生—肿瘤]