口腔黏膜组织结构及DNA损伤修复功能的增龄性变化  

A Study on Age-related Changes of Histological Structure and DNA Repair Capacity in Oral Mucosa

作　　者：周红梅[1] 杨娅[1] 周敏[1] 郑晓晖[2] 王智[1] 何志秀[3] 李秉琦[4] 

机构地区：[1]四川大学华西口腔医院口腔黏膜科,610041 [2]四川大学华西口腔医院颌面外科 [3]四川大学华西口腔医院病理科 [4]四川大学华西口腔医院口腔黏膜科(郑晓晖,610041

出　　处：《华西口腔医学杂志》2003年第3期177-179,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (编号 30 0 70 81 5)

摘　　要：目的　研究口腔黏膜组织结构及DNA损伤修复功能 (以P5 3蛋白表达为指标 )的增龄性变化 ,探讨老年人好发口腔鳞状细胞癌的可能机制。方法　以不同年龄健康人的正常口腔黏膜组织作为实验对象 ,分别采用常规HE染色法和免疫组织化学技术 (SP法 ) ,研究各组口腔黏膜组织结构特征及P5 3蛋白表达情况 ,并用半定量法对SP染色结果进行判定。结果　①随年龄的增长 ,口腔黏膜组织结构发生系列变化 ;②随年龄增长 ,口腔黏膜组织中P5 3阳性表达率增高 ,染色分值也呈上升趋势 (P <0 0 5 )。结论　口腔黏膜组织结构及DNA损伤修复功能的增龄性变化可能是老年人口腔上皮易发生癌变的原因之一。Objective\ To investigate the age_related changes of histological structure and DNA repair capacity (P53 protein expression) in oral mucous membrane and try to explain why the elders are prone to oral squamous cell carcinoma.Methods\ The routine HE staining and an immunohistochemical technical (SP method) were used respectively to study the changes of histological structure and P53 expression in normal oral mucosa of different age groups. The SP staining results were evaluated by semi_quantity method.Results\ ①With aging, a series of histological changes were observed in the oral mucosa; ② With aging, an elevating tendency of P53 protein level emerged in the oral mucosa (P<0 05).Conclusion\ The sensitivity to carcinogenic agents in elder people may be accentuated by the age_related histological changes and DNA repair capacity changes of the normal oral mucosa.\;

关 键 词：口腔黏膜 组织结构 DNA损伤修复功能 增龄性变化 

分 类 号：R780.2[医药卫生—口腔医学]