内源性高甘油三酯血症患者血浆VLDL、LDL及HDL对血凝的影响  

Effects of oxidative modification plasma very low density lipoprotein,low density lipoprotein and high density lipoprotein on blood coagulation in endogenous hypertriglyceridemia

作　　者：邓祖跃[1] 刘秉文[1] 田汉文[2] 刘宇[1] 白怀[1] 

机构地区：[1]四川大学华西基础医学与法医学院载脂蛋白研究室,成都610041 [2]四川大学华西基础医学与法医学院药理教研室,成都610041

出　　处：《四川生理科学杂志》2003年第1期15-19,共5页Sichuan Journal of Physiological Sciences

基　　金：国家重点基础研究发展计算 973规划项目编号G2 0 0 0 0 5 6 90 0

摘　　要：目的 :研究内源性高甘油三酯血症 (HTG)患者血浆极低密度脂蛋白 (VLDL)、低密度脂蛋白(LDL)及高密度脂蛋白 (HDL)是否发生了氧化修饰及其对血凝的影响。方法 :对 2 1例内源性高甘油三酯血症患者与 2 1例年龄性别相近的正常人的血脂、脂质过氧化物进行了分析。用一次性密度梯度超速离心法分离血浆VLDL、LDL及HDL ,测定这三种脂蛋白的 2 3 4nm光吸收、相对电泳迁移率 (REM )和硫代巴比妥酸反应物质(TBARS) ,分别将这三种脂蛋白加入由正常人新鲜混合血浆构成的反应系统中 ,按试剂盒分别测定凝血酶原时间 (PT)及活化部分凝血酶原时间 (APTT)。结果 :内源性HTG患者血浆TG含量平均升高 2 .73倍 ,HDLC下降 1 .71倍 ,同时LPO升高 1 .2 2倍 ;HTG组VLDL、LDL及HDL的REM、2 3 4nm光吸收值、TBARS含量均较对照组显著增加 (P <0 .0 1 ) ,表明内源性HTG患者血浆VLDL、LDL及HDL均发生了氧化修饰生成Ox VLDL、Ox LDL及Ox HDL。PT及APTT在分别加入HTG组的VLDL、LDL及HDL后均比加入相应正常组脂蛋白明显缩短 (P均 <0 .0 5 )。相关分析表明 ,HTG组血浆VLDL及HDL相对电泳迁移率 (REM )与PT呈负相关 (P <0 .0 1 )。结论 :HTG患者血浆VLDL、LDL及HDL发生了氧化修饰 ,并使PT及APTT明显缩短。Objective: To study whether plasma very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) were oxidatively modified in endogenous hypertriglyceridemia and effects of HTG VLDL, LDL and HDL on blood coagulation in vitro. Methods: Plasma VLDL, LDL and HDL were isolated by density gradient ultracentrifugation method . Human plasma triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDLC) were measured by enzyme method. The oxidative modification of LDL, VLDL and HDL was identified by agarose gel relative electrophoretic mobility (REM), absorbance at 234nm (CD) and fluorescence of thiobarbituric acid reaction substances (TBARS). With the reaction system which consisted of mixed fresh normal plasma, prothrombin time (PT) and activated partial thrombplastin time (APTT) were measured according to the direction of the kits. Results: the plasma TG, TBARS level in HTG group were 1.6 and 0.4 times of the control group ,respectively (P<0.01). The plasma HDLC in HTG group was 32% lower than that of the control group (P<0.01). The relative electrophoretic mobility (REM), absorbance at 234nm and TBARS of VLDL, LDL and HDL in HTG group were significantly higher than that of the control group (P<0.01). The PT and APTT of VLDL , LDL and HDL in HTG group were significantly shorter than that of the control group (P<0.05). The correlation analysis indicated that electrophoretic mobility of VLDL and HDL in HTG group was negatively correlated with PT (P<0.01). Conclusion: Oxidative modification of plasma very low density lipoprotein, low density Lipoprotein, high density lipoprotein are occurred in endogenous hypertriglyceridemia in vivo and LDL and HDL enhance the activity of blood clotting system in vitro.

关 键 词：内源性高甘油三酯血症 血浆 VLDL LDL HDL 血凝 影响 

分 类 号：R589.2[医药卫生—内分泌]