三氧化二砷及干扰素联用对K562及K562/ADM耐药细胞系的作用  

Effect of Arsenic Trioxide Combind with Interferon in K562 and K562/ADM Cell Lines

作　　者：苏颖[1] 邹长棪[1] 陈增[1] 林华妹[1] 林可焴[1] 

机构地区：[1]福建省肿瘤医院放射生物研究室,福州350014

出　　处：《福建医科大学学报》2003年第2期155-157,共3页Journal of Fujian Medical University

基　　金：福建省卫生厅科研基金资助项目 ( 960 3 8)

摘　　要：目的　　研究三氧化二砷 (As2 O3)对 K5 6 2及其耐药细胞 K5 6 2 / ADM细胞株几种耐药机制的作用 ,以及干扰素 (IFNα- 2 b)与之联用的效应。方法　　采用免疫细胞化学染色及 TU NEL 原位末端标记检测不同浓度 As2 O3作用不同时间或与 IFNα- 2 b联用 ,对 K5 6 2及 K5 6 2 / ADM细胞相关耐药基因蛋白 (P- gp、GST-π、Bcl- 2 )表达的影响及凋亡诱导作用。　结果　 K5 6 2、K5 6 2 / ADM细胞表面 P- gp蛋白表达无明显改变 ;As2 O3(5 ,10 ,2 0 μm ol/ m L)作用 72 h可降低 K5 6 2、K5 6 2 / ADM细胞 GST- π的表达 ,与对照组比较 P<0 .0 5 ;As2 O3抑制 K5 6 2、K5 6 2 / ADM细胞 Bcl- 2蛋白的表达 ,诱导细胞凋亡 ,并有计量时间效应 ;IFNα- 2 b与 As2 O3联用可增强对 K5 6 2细胞的作用 ,对 K5 6 2 / ADM细胞未见明显增强效应。　结论　As2 O3具有抑制 K5 6 2、K5 6 2 / ADM细胞 GST- π、Bcl- 2蛋白的表达及诱导细胞凋亡作用 ;对 P- gp蛋白表达无明显影响 ;IFNα- 2 b可增强其对 K5 6Objective To study the effect of arsenic trioxide(As\-2O\-3) combind with interferon(IFNα\|2b) on multidrug\|resistance of K562 and K562/ADM cell lines. Methods Immunocytochemistry and terminal deoxynucleotidyl transferase nick end labeling(TUNEL) were used to detect the expression of P\|gp, GST\|π and Bcl\|2, and the induction of apoptosis by As\-2O\-3 at different doses and time or combined with IFNα\|2b. Results The expression of P\|gp did not change; the expression of GST\|π was decreased(P<0 05 vs contrast) following treatment 72 h with arsenic trioxide(5, 10, 20 μmol/mL); the expression of Bcl\|2 was decreased and the number of apoptotic cells increased, which enhanced with the increment of arsenic trioxide dosage and time, the effect was more prominent in IFN combined with As\-2O\-3 for K562 cell. Conclusion Arsenic trioxide may suppress the expression of GST\|π, Bcl\|2 and induce apoptosis of K562, K562/ADM cell lines, and did not affect expression of P\|gp. IFN might enhance the effect of As\-2O\-3 on K562 cells.

关 键 词：砷剂 干扰素 抗药性 多药 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]