利用凝胶介质提高古菌DNA结合蛋白晶体的衍射分辨率  

Improvement on Diffraction Resolution of an Archaeon DNA Binding Protein by Gel Medium

作　　者：王刚刚[1] 杨海涛[1] 饶子和[1] 

机构地区：[1]清华大学生物科学与技术系,教育部蛋白质科学重点实验室,北京100084

出　　处：《生物化学与生物物理进展》2003年第3期471-473,共3页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划项目"973" (G19990 75 60 2 )~~

摘　　要：在凝胶介质中结晶了极端嗜酸热菌Sulfolobus shibataeDNA结合蛋白Ssh10b ,与没有凝胶介质的条件下相比 ,晶体的出晶速度减缓 ,晶体的外形变得更规则 ,晶胞的C轴变短 ,晶体衍射分辨率从 0 65nm提高到了0 3 5nm .A small DNA-binding protein (Ssh10b) from the hyperthermophitic archaeon Sulfolobus shibatae was crystallized. The crystals could only diffract to 0.65 nm. And gel medium was used to improve the diffraction resolution. Gel adding led to slower speed of crystallization, change of crystal morphology and shortening of C axis of unit cell. The crystals grown in gel medium could diffract to 0.35 nm, and a set of data was collected.

关 键 词：凝胶介质 古菌 DNA结合蛋白 分辨率 蛋白晶体 晶体衍射 

分 类 号：Q51[生物学—生物化学] Q6－33