溶脲脲原体与人精子膜蛋白交叉反应抗原的研究  

Study on the Cross-Reactive Antigens between Ureaplasma Urealytium and Human Sperm Membrane Proteins

作　　者：石建莉[1] 鲁梅格[1] 王一飞[1] 王益清[2] 朱洪[2] 周元聪[2] 徐晨[1] 

机构地区：[1]上海第二医科大学组织胚胎学教研室,上海200025 [2]中国科学院上海生命科学院生物化学与细胞生物学研究所蛋白质组学重点实验室,上海200031

出　　处：《生殖与避孕》2003年第3期153-157,T004,共6页Reproduction and Contraception

基　　金：上海市教委曙光计划 (No.99SG4 2 )资助

摘　　要：目的 :研究溶脲脲原体 ( Ureaplasma urealyticum,UU)与人精子膜之间的交叉反应抗原。方法 :将从不育患者精液中分离、纯化等获取的 UU蛋白质与人精子膜蛋白进行十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳 ( SDS- PAGE) ,再将电泳条带转移到硝酸纤维素膜上 ,用兔抗人精子膜抗血清及羊抗兔 Ig G- HRP进行 Western blot反应。结果 :Western blot证实 UU中 61 k D、5 0 k D和 2 7k D蛋白质组份与精子膜蛋白具有相似抗原性。结论 :UU与人精子膜之间存在交叉反应抗原 ,这可能与临床上 UU感染不育患者血清和 /或精液中抗精子抗体 ( As Ab)Objective:To investigate on the cross reactive antigens between Ureaplasma urealyticum(UU) and human sperm membrane proteins. Methods:Proteins were separated by SDS PAGE and transferred to nitrocellulose membranes. Then Western blot analysis was performed. Results:Cross reactive antigens of 61, 50 and 27 kD were identified. Conclusion: The cross reactive antigens between UU and human spermatozoa may be related to the phenomenon that infertile men who are infected with UU display higher titer of serum and/or semen antisperm antibodies (AsAb) compared to non infected men.

关 键 词：溶脲脲原体 人精子膜蛋白 交叉反应抗原 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]