小鼠卵透明带3(mZP3)质粒DNA体外瞬时表达的研究  

Assay of Transient Expression of Plasmid Murine Zona Pellucida 3 DNA In vitro

作　　者：李再新[1] 张富春[1] 金华利[1] 王艳萍[1] 任智慧[1] 王宾[1] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室

出　　处：《地方病通报》2003年第2期5-8,共4页Endemic Diseases Bulletin

基　　金：国家自然科学基金 (39960 0 79);"863"高技术青年基金资助项目

摘　　要：为探讨小鼠卵透明带 3 (mZP3 )质粒DNA在真核细胞中的表达效率 ,筛选出高效表达mZP3的质粒 DNA ,提高DNA疫苗对小鼠的免疫不育效果 ,本研究采用阳离子多聚糖 纳米颗粒Puchio为转染介质 ,将mZP3质粒DNA转染真核细胞COS 7,用半定量 PCR检测mZP3的特异性mRNA表达。结果显示 :Puchio与pcDNA3 mZP3紧密结合 ,在COS 7细胞中有mZP3基因的特异性mRNA表达 ;Puchio的介导可能提高真核细胞的转染效率 ,有助于通过真核细胞的瞬时表达系统筛选出用于小鼠免疫不育的DNA疫苗 ,为深入开展鼠卵透明带To investigate mRNA expression of murine Zona Pellucida 3 gene in the eukaryocytes and to gain effective plasmid to enhance the immune infertility for mZP3 DNA vaccine, the plasmid pcDNA mZP3 was transfected into eukaryocyte COS 7 cells with the transfection medium Puchio. The special mRNA level of pcDNA3 mZP3 was assayed by SqRT PCR (Semi quantitative Reverse Transcription and Polymerase Chain Reaction). The results showed that the pcDNA3 mZP3 was closely combined with Puchio to form the nanometric complex in the transfection. The special mRNA bend was presented in the assay of SqRT PCR. It was indicated that Puchio could improve the function of transfection for eukaryocytes. That the DNA vaccine of infertility screened through the transient expression system of the eukaryocytes could be a basis of the further exploitations of mZP3 DNA vaccine to reduce a plague of murine.

关 键 词：鼠卵透明带3(mZP3) Puchio 转染 半定量检测 

分 类 号：Q186[生物学—普通生物学] R－332[医药卫生]