A型产气荚膜梭菌α毒素全基因的分子克隆与核苷酸序列分析  

Cloning and sequencing of α-toxin gene of Clostridium perfringens type A

作　　者：林明辉[1] 荫俊[1] 王慧[1] 宋伟[1] 邢洪光[1] 侯晓军[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《生物技术通讯》2003年第3期180-181,共2页Letters in Biotechnology

摘　　要：应用PCR技术,从A型产气荚膜梭菌菌株NCTC64609中扩增出A型产气荚膜梭菌α毒素全基因(cpa1229基因)并将其克隆至pMD18-T载体中。经转化、IPTG/X-gal选择培养,提取质粒,PCR和EcoRI/PstI双酶切鉴定,筛选阳性重组克隆。经核苷酸序列分析证实,cpa1229基因阅读开放框架由1194bp组成。经GenBank检索对照分析,cpa1229基因序列与国外文献报道者同源性达98.3%,表明本实验所克隆的cpa1229基因即为A型产气荚膜梭菌α毒素基因。The complete gene ofα-toxin (cpa1229gene)was amplified from the strain NCTC64609of Clostridium perfringens type A by PCR,and then was cloned into a clone vector pMD18-T.By PCR and Eco RI /PstI digestion,the gene was identified.After being sequenced,the cpa1229gene was known to be consist of1194bp enconding398amino acid residues.After being analyzed by computer,the gene was known to be consistent with the publishedα-toxin gene of the foreigners up to98.3%.

关 键 词：A型产气荚膜梭菌 Α毒素 基因 分子克隆 核苷酸序列 

分 类 号：Q933[生物学—微生物学] Q785