简便实用的琼脂糖凝胶回收DNA片段方法  

A convenient and practical method of recovering DNA band from the agarose gel

作　　者：庄飞云[1] 茅淑敏 娄群峰[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院作物遗传与种质创新国家重点实验室,南京210095 [2]浙江森和种业股份有限公司,杭州310021

出　　处：《生物技术通讯》2003年第3期202-203,共2页Letters in Biotechnology

基　　金：国家自然科学基金资助项目(30170644);教育部科学技术研究重点项目(重点01097)

摘　　要：介绍一种简便实用的DNA片段回收方法,与以前所报道的DEAE-纤维素膜电泳法、透析袋电洗脱法、低融点琼脂糖凝胶法、凝胶冻融法等相比,所需器材简单、操作简便、回收率高、成本低。回收的DNA片段在进一步克隆和测序中表现出较好的效果。The method of recovering DNA band introduced in this paper was convenient and practical.Compared with the DEAE-cellulose membrane electrophoretic method,dialysis membrane electroelution method,low gelling tem-perature agarose gels method,and gel freezing and thawing method,it was simple requirement of equipments,easy operation,high rate of recovery,and low cost.The results showed better in the cloning and sequencing with the re-covered DNA band.It was an experiment method fit for both research and education.

关 键 词：琼脂糖凝胶 回收 DNA片段 分子生物学实验 电泳 

分 类 号：Q503[生物学—生物化学]