汉滩病毒M片段的核苷酸序列变异和系统发生树分析  

Genetic Diversity of Partial M Fragment of Hantaan Virus Hubei Strain and Analysis of Phylogenetic Tree

作　　者：丁晓华[1] 杨占秋[1] 肖红[1] 陈晓湘 徐芳玲[1] 侯炜[1] 文利[1] 

机构地区：[1]武汉大学医学院病毒学研究所,武汉430071

出　　处：《病毒学报》2003年第2期169-172,共4页Chinese Journal of Virology

基　　金：国家自然科学基金课题(39870659)

摘　　要：The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 540-1,244 nucleotides of M fragment of Hantaan virusThen the amplified PCR products were sequenced directlyThe sequencing results demonstrated that there was 84% identity between sample 613 and HV114 virus strain,but was 99% between sample 613 and HTN76-118 strainHowever,in sample 226,there was 95% sequence identity with HV114,82% with HTN76-118The results of phylogenetic tree analysis showed that HV613 was located in the same linage with HTN76-118,the HV226 was in the same linage with HV114 and A9The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 540-1,244 nucleotides of M fragment of Hantaan virusThen the amplified PCR products were sequenced directlyThe sequencing results demonstrated that there was 84% identity between sample 613 and HV114 virus strain,but was 99% between sample 613 and HTN76-118 strainHowever,in sample 226,there was 95% sequence identity with HV114,82% with HTN76-118The results of phylogenetic tree analysis showed that HV613 was located in the same linage with HTN76-118,the HV226 was in the same linage with HV114 and A9 strains

关 键 词：汉滩病毒 M片段 核苷酸 变异 系统发生树 序列分析 

分 类 号：R373.32[医药卫生—病原生物学]