直肠癌和直肠正常组织的傅里叶变换红外光谱研究  

FTIR Spectroscopic Study of Normal and Malignant Tissues of Rectum

作　　者：王凡[1] 凌晓峰[2] 杨展澜[1] 徐智[2] 任予[3] 李维红[1] 翁诗甫[1] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院,化学生物学系北京100871 [2]北京大学第三医院,北京100083 [3]西安交通大学第一医院,陕西西安710061

出　　处：《光谱学与光谱分析》2003年第3期498-501,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (2 9671 0 0 2 ;39730 1 60;2 0 0 2 30 0 5);国家重点基础研究发展规划项目G1 9980 61 30 7;科技部前期研究专项 2 0 0 2CCA0 1 90 0资助

摘　　要：利用傅里叶变换中红外 (FTIR)光纤和ATR技术研究了 2 1例直肠癌病人的正常和癌变组织。对319张光谱进行数据分析的结果表明 ,五个相对峰高 (I2 873 I2 854,I1 74 5 I1 6 43,I1 458 I1 40 0 ,I1 2 4 0 I1 31 5和I1 1 6 2 I1 0 82,单位为cm- 1 )的差别可用于区分直肠癌和直肠正常组织。用这一差别分析了距离直肠癌 1cm处组织的光谱 ,发现该组织处于正常和癌变的过渡状态。Fourier transform infrared (FTIR) spectroscopy was used to study the normal and malignant tissues of rectum. The FTIR spectra of tissues were measured by Nicolet Magna IR-750 spectrometer equipped with mid-infrared fiber optics. The results show that the intensity ratios of five pairs of bands can be used to distinguish the normal and malignant tissues. In the spectra of malignant tissues the relative intensities ratios of I-2873/ I-2852 and I-1312/ I-1245 were higher than those of normal ones; however, the ratios of I-1 745 / I-1 643, I-1458/I-1400 and I-1162/I-1082 were lower. These results, proved by data-analysis of spectra from 21 patients, were generally shown in the spectra of rectum tissues. The study of near-malignant tissues (one centimeter beside the malignant point) of rectum by FTIR shows that these ratios of bands were in a transient state between normal and malignant rectum tissues.

关 键 词：直肠癌 直肠正常组织 傅里叶变换红外光谱 FTIR 癌变组织 数据分析 癌症诊断 

分 类 号：R735.37[医药卫生—肿瘤] O657.33[医药卫生—临床医学]