汞与硒在不同汞暴露水平地区猪肝脏和肾脏蛋白质中的分布  

Distribution of Mercury and Selenium in the Proteins of Porcine Liver and Kidney Under Different Mercury Exposure Level

作　　者：赵九江[1] 章佩群[1] 陈春英[1] 柴之芳[1] 瞿丽雅 李梅 

机构地区：[1]中国科学院高能物理所核分析技术开放实验室,北京918信箱北京100039 [2]贵州环境科学研究设计院,贵阳550002

出　　处：《中国生物化学与分子生物学报》2003年第3期377-382,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金 (No.1993 5 0 2 0No .2 0 0 710 3 2 );中国科学院重要方向性项目 (No .KJCX2 N10 1)资助~~

摘　　要：通过对贵州万山汞污染地区及北京地区猪肝脏和肾脏组织上清液进行凝胶过滤色谱分离(SephadexG 10 0 ) ,随后用原子荧光法测定它们蛋白质组分中汞和硒的含量 ,研究在汞暴露水平不同状态下微量元素汞和硒在动物体蛋白质分子水平上的分布 .发现这两个地区猪肝脏和肾脏组织上清液蛋白质组分中汞和硒的分布模式有明显差异 .贵州万山汞污染地区猪肝脏上清液中汞浓度比北京地区高 ,硒浓度也相应高 ,且前者与高分子量和低分子量蛋白结合的硒均明显高于后者 ;而北京地区猪肝脏上清液中的硒主要以与高分子量蛋白结合的形式存在 .贵州汞污染地区猪肝脏上清液中汞主要与高分子量蛋白结合 ,而北京地区猪肝脏上清液中汞则分布较为均匀 .贵州万山地区猪肾脏上清液中 ,含硒峰在高分子量蛋白区和低分子量区都有分布 ;而北京地区猪肾脏上清液中 ,硒则主要集中分布于高分子量蛋白范围 .这两个地区猪肾脏上清液中都有分子量约为 11kD的金属硫蛋白 (MT)存在 ,北京地区猪肾脏上清液中汞主要以与金属硫蛋白结合的形式出现 ,而贵州万山地区猪肾脏上清液中的汞除与金属硫蛋白结合外 ,尚有相当大部分是以与高分子量蛋白结合的形式存在 .研究结果表明 ,由于这两个地区汞暴露水平的差异 ,不仅使这两地区猪肝。The mercury and selenium in cytosolic proteins porcine liver and kidney from Guizhou and Beijing were determined by atomic fluorescence spectrometry after separation with Sephadex G\|100 gel filtration chromatography. The results showed that the distribution patterns of selenium and mercury in the cytosolic proteins from the two area samples were different. Selenium was mainly bound to the cytosolic proteins with high molecular weight in Beijing porcine liver, while it was also present with low molecular weight fractions besides being bond to the high molecular weight proteins for the Guizhou one. Mercury was mainly bound to the proteins with high molecular weight for the Guizhou porcine liver. Se\|containing components existed in the fractions of both high and low molecular weight for the Guizhou porcine kidney, while they were only present in the fractions of high molecular weight for Beijing one. Mercury was mainly in the form of metallothioneins (MTs) in the cytosol of Beijing porcine kidney. On the contrary, it was mainly bound to the proteins of higher molecular weight besides to MTs for the Guizhou one. The present results indicate that the different level of mercury exposure results in not only the different contents of selenium and mercury in the cytosolic proteins of porcine liver and kidney from the two areas, but also their different distribution patterns, suggesting that there is a certain correlation between the two elements in the metabolism of organisms.

关 键 词：汞 猪 肝脏 肾脏 蛋白质 分布 硒 凝胶过滤色谱分离 原子荧光法 

分 类 号：Q51[生物学—生物化学] X132[环境科学与工程—环境科学]