γ射线辐照和无性系变异相结合诱发创造优异恢复系突变体R3027  

AN ELITE RESTORER MUTANT R3027 INDUCED BY THE INTEGRAL APPLICATION OF γ-RAYS IRRADIATION AND SOMACLONAL VARIATION TECHNIQUES

作　　者：沈圣泉[1] 吴殿星[1] 高明尉[1] 夏英武[1] 舒庆尧[1] 

机构地区：[1]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《核农学报》2003年第3期165-170,共6页Journal of Nuclear Agricultural Sciences

基　　金：科技部"九五"生物技术攻关项目和浙江省"8812"项目

摘　　要：对中籼材料 3 0 2 7的幼穗先经 1 5Gy的6 0 Coγ射线辐照处理 ,而后接种于N6 培养基上进行愈伤组织诱导 ,MS培养基上分化诱发产生了一系列的体细胞无性系变异株 ,从中筛选出早熟恢复系突变体R3 0 2 7。与原亲本 3 0 2 7相比 ,突变体R3 0 2 7的多个农艺性状和配合力均明显改良。与细胞质雄性不育系Ⅱ 3 2A配组 ,成功选育单产最高达 1 0 477kg hm2 的杂交稻新组合Ⅱ优 3 0 2 7。Series of somaclonal variants were induced from young inflorescence culture of single season indica rice 3027,which was irradiated by 15Gy γ\|rays and then transferred onto fresh N\-6 medium for callus formation and MS medium for shoot differentiation.One early maturing restorer mutant,named as R3027,was identified and found to possess the obviously improved multiple agronomic traits and combined ability in comparison with the original parent 3027.A new hybrid rice cultivar,Ⅱ you 3027,with the highest yield potential of 10477kg/hm\+2 was successfully bred from the cross between the cytoplasmic male sterile line Ⅱ\|32A and mutant R3027.This new cultivar was officially released in April,2000 in Zhejiang province,and its accumulative planting areas reached 200khm\+2.

关 键 词：水稻 突变体R3027 恢复系 γ射线 体细胞无性系变异 诱变育种 特征特性 配合力 应用 

分 类 号：S33[农业科学—作物遗传育种]