水稻胚乳突变体的诱发及其微卫星分子标记鉴定  

INDUCTION OF ENDOSPERM MUTANTS AND CHARACTERIZATION OF THE MICROSATELLITE MARKER IN RICE( Oryza sativa L.)

作　　者：吴殿星[1] 段智英[1] 舒庆尧[1] 沈圣泉[1] 韩娟英[1] 夏英武[1] 

机构地区：[1]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《核农学报》2003年第3期175-178,共4页Journal of Nuclear Agricultural Sciences

基　　金：国际原子能机构技术合作项目CPR 0 5 0 13;浙江省重大项目"94 10"资助

摘　　要：用 3 50Gy6 0 Coγ射线辐照高表观直链淀粉含量 (apparentamylosecontent,AAC)早籼稻新品种金早 97 47,筛选鉴定出 4个暗胚乳突变体和 2个云雾状胚乳突变体。AAC测定表明 ,暗胚乳和云雾状胚乳突变的AAC明显比原亲本低。以Wxup2 485为引物 ,发现两类胚乳突变的Wx基因微卫星分子标记相同 ,与原亲本显著不同 ,前者为 (CT) 1 8 (CT) 1 8,后者为 (CT) 1 1 (CT) 1 1。Four dull endosperm mutants and two misty endosperm mutant of rice ( Oryza sativa L.) were induced from a high apparent amylose early indica rice\_\_Jinzao 97\|47 with 350 Gy γ\|rays irradiation. AAC of the endosperm mutants was significantly lower than that of the original parent. Using the Wxup2/485 as primer,it was found that both types of endosperm mutants had the same microsatellite markers of Wx gene in (CT)18/(CT)18,which was different from that of the original parent in (CT)11/(CT)11.

关 键 词：水稻 胚乳突变体 Wx基因微卫星分子标记 鉴定 淀粉 品质育种 Γ射线辐照 

分 类 号：S511[农业科学—作物学]