裙带菜基因工程选择标记的研究  

Study on Selectable Markers in Genetic Engineering of Undaria pinnatifida

作　　者：于道展[1] 杨玲玲[2] 姜鹏 秦松[1] 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所 [2]中国海洋大学生命学院,青岛266003

出　　处：《高技术通讯》2003年第6期74-77,共4页Chinese High Technology Letters

基　　金：中国科学院知识创新工程(KZCX3-SW-215)资助项目。

摘　　要：研究了裙带菜幼孢子体对10种选择试剂的敏感性。结果发现裙带菜幼孢子体对氯霉素、潮霉素敏感,对除草剂草丁膦极敏感(2天的LD50为8.57mg/L)。根据统计学的要求得出了这3种选择试剂对裙带菜幼孢子体的半致死剂量和95％可信限。本文的结果能为裙带菜基因工程选择标记的建立提供依据。The sensitivities of young sporophytes of Undaria pinnatifida to ten selective reagents including chloram-phenicol, hygrcmycin, kanamycin, G418, zeocin, penicillin G, streptomycin, lincomycin, basta and amethopterin were tested. Young sporophytes of U. pinnatifida were found sensitive to chloramphenicol and hygromycin, and very sensitive to basta. LD50 and 95% confidence ranges of these three reagents were calculated separately. The results provide important data in developing selectable markers in genetic engineering of Undaria .

关 键 词：裙带菜 幼袍子体 基因工程 选择标记 氯霉素 潮霉素 草丁膦 敏感性 

分 类 号：Q943[生物学—植物学]