节旋藻FACHB341 Rubisco基因部分序列的克隆和分析  

Cloning and Sequence Analysis of Rubisco Gene from Arthrospira platensis FACHB341

作　　者：刘金姐[1] 茅云翔[1] 臧晓南[1] 隋正红[1] 张学成[1] 

机构地区：[1]中国海洋大学海洋生命学院,青岛266003

出　　处：《高技术通讯》2003年第6期87-93,共7页Chinese High Technology Letters

基　　金：863计划(863-819-04-10)资助项目。

摘　　要：以节旋藻FACHB341为材料,对所克隆Rubisco基因进行了核苷酸序列测定和分析,由此推导出相应的氨基酸序列,并与部分其他蓝藻的同源基因进行了同源性分析。结果表明:所克隆DNA片段包含Rubisoo大小亚基基因部分序列及rbcX基因序列,长度为2073 bp,其中rbcL和rbcX基因之间存在两个转录茎环结构;大小亚基酸性氨基酸和碱性氨基酸的比例分别为13.14％和14.51％,疏水氨基酸的比例为42.16％;rbcL核苷酸序列与集胞藻PCC6803、Prochlorothrix hollandica、聚球藻PCC6301、Agmenellum quadruplicatum和鱼腥藻PCC7120同源序列的相似性分别为91.7％、79.9％、74.8％、77.2％和76.1％;rbcS核苷酸序列与鱼腥藻PCC7120同源序列的相似性为67.6％,而与PCC6301和集胞藻PCC7002同源序列的相似性分别为30.1％和63.8％。A fragment of Rubisco gene is cloned, sequenced and analyzed from a cyanobacterium, Arthrospira (Spir-ulina) platensis FACHB341. The length of the fragment is 2073 bp, including partial sequence of rbcL gene, rbcX gene and rbcS gene. There are two transcription stem-loop termination structures downstream of the 3' sequence of rbcL gene. The deduced amino acid sequence of rbcL and rbcS includes 13.14% acidic amino acid and 14.51 % aliphatic amino acid, the hydrophobic amino acid is 42.16% . The similarity of rbcL gene with homologous gene sequences in Agmenellum quadruplicatum, Synechocystis PCC6803, Synechococcus PCC6301, Prochlorothrix hollandica and Anabaena PCC7120 is 77.2% , 81.7% , 74.8% , 79.9% and 76.1% , respectively. The similarities of rbcS gene with homologous gene in four Anabaena sp. sequences are all 67.6% , but it is 30.1% with one Synechococcus sp. and 63.8% with the other one.

关 键 词：节旋藻 基因序列 克隆 “l 5-二磷酸核酮糖羧化酶/加氧酶” 核苷酸 同源性分析 氨基酸 RUBISCO基因 密码子 

分 类 号：Q943[生物学—植物学]