提高新月弯孢霉原生质体再生率的研究  

Protoplast Formation and Regeneration of Curvularia Lunata

作　　者：卢文玉[1] 郭亚文[1] 龚未[1] 王敏[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院制药工程系,天津300222

出　　处：《药物生物技术》2003年第3期141-143,共3页Pharmaceutical Biotechnology

基　　金：天津市重大科技攻关项目 (0 2 3 180 2 11);教育部重点科技研究项目 (天津 0 10 0 4)~~

摘　　要：报道了氢化可的松生产菌新月弯孢霉的原生质体制备与再生条件。将培养 2 2h的菌丝体用0 .3mol L的二硫苏糖醇处理 2 0min ,0 .6mol L的KCl作为渗稳剂 ,30℃下经 0 .5 %新生酶 2 34和 1%溶壁酶混合液(pH5 .6 )酶解 3.5h ,原生质体释放量达到最大值 8.15× 10 6 个 ml,再生率为 2 3.0 7%。再生菌株菌落形态不变 ,保留了对底物RSA的羟化能力。Protoplasts were obtained from Curvularia lunata , producting strains of hydrocortisone. Mycelia of Curvularia lunata cultured for 22h were harvested and suspended in 0.3mol/L DTT for 20min. The protoplasts yield achieved 8.15×10 6 /ml under 30℃ with 0.5% novozym and 1% lywallzyme(pH5.6) using 0.6mol/L KCl as osmotic stabilizer in 3.5h. About 23.07% of protoplasts were capable of regenetation, meanwhile the morphology and 11β hydroxylation ability of colonies regenerated strains were the same with the parent strain.

关 键 词：新月弯孢霉 原生质体 氢化可的松 

分 类 号：TQ92[轻工技术与工程—发酵工程]