典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟Na^+-K^+-ATPase的抑制作用  

Inhibition of α-Terthienyl on Na^+-K^+-ATPases Activity inHelicoverpa armigera and Ostrinia furnacalis Larvae

作　　者：蒋志胜[1] 颜增光[1] 何佳[1] 杜育哲[2] 尚稚珍[1] 

机构地区：[1]南开大学元素有机化学研究所,元素有机化学国家重点实验室,天津300071 [2]南开大学生命科学学院生物系,天津300071

出　　处：《农药学学报》2003年第2期47-52,共6页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金 (3990 0 0 94 );天津市自然科学基金 (0 0 370 1311)资助课题

摘　　要：以棉铃虫 H elicoverpa armigera和亚洲玉米螟 Ostrinia furnacalis为试虫 ,建立 Na+- K+-ATPase最佳反应系统 ,研究典型光活化毒素 α-三噻吩 (简称 α- T)对 Na+- K+- ATPase的影响。棉铃虫 Na+- K+- ATPase活力最佳测试条件为酶源蛋白浓度 6μg/ m L ,反应温度 35～ 4 0℃ ,反应时间6min;亚洲玉米螟则为酶源蛋白浓度 8μg/ m L,反应温度 35℃ ,反应时间 6min。近紫外光照 (30 0～4 0 0 nm )对棉铃虫和亚洲玉米螟离体 Na+- K+- ATPase活力基本没有影响 ,但对活体活力有很强的抑制作用。光照和无光照条件下 ,α- T对两种昆虫离体和活体 Na+- K+- ATPase活力均有不同程度的抑制作用。光照组 α- T对亚洲玉米螟 Na+- K+- ATPase的抑制率高于无光照组 ,且处理浓度或剂量越高 ,其抑制率越大 ;对棉铃虫 Na+- K+-The optimal reaction system of Na +-K +-ATPase from the larval heads of Helicoverpa armigera and Ostrinia furnacalis was set up, respectively. The optimal reaction condition for H. Armigera was 6 μg/mL for protein concentration, temperature 35~40℃ and time 6 min, for O. furnacalis was 8 μg/mL for protein concentration, temperature 35℃ and time 6 min. Under those optimized conditions, effects of α-terthienyl (α-T), a typical photoactivated insecticide, on Na +-K +-ATPase in insect pests were studied. The results showed that Na +-K +-ATPase activity in vivo was significantly inhibited by the near ultraviolet irradiation (300~400 nm), which almost had no effect on that in vitro. However, Na +-K +- ATPase activity in two insect pests after treated by α-T was inhibited both in vivo and in vitro whether irradiation or not. Inhibition rate of Na +-K +-ATPase activity from larvae of O. furnacalis in light group was higher than that in non-light group, and also showing good response-dosage relationship. This suggested that Na +-K +-ATPase might be involved in the mechanism of the action mode of α-T.

关 键 词：NA^+-K^+-ATPASE α-三噻吩 棉铃虫 亚洲玉米螟 

分 类 号：Q965[生物学—昆虫学]