麦洼牦牛和九龙牦牛乳蛋白遗传多态性的研究  

GENETIC POLYMORPHISMS OF MILK PROTEIN IN MAIWA AND JIULONG YAK

作　　者：毛永江[1] 钟光辉[2] 郑玉才[2] 彭先文[3] 杨章平[1] 王永[2] 江明峰[2] 

机构地区：[1]扬州大学畜牧兽医学院动物科学系,江苏扬州225009 [2]西南民族大学生命技术学院,四川成都610041 [3]湖北省农科院畜牧兽医研究所,湖北武汉430209

出　　处：《扬州大学学报（农业与生命科学版）》2003年第2期7-10,20,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(39870607)

摘　　要：利用聚丙烯酰胺凝胶电泳对109头麦洼牦牛和100头九龙牦牛乳蛋白多态性进行分型,并采用最小二乘法分析乳蛋白多态性与泌乳性能及乳蛋白组分之间的相关性。结果表明:β CN、κ CN和α La呈单态,基因型分别为AA、BB和BB型;而αs1 CN和β Lg呈多态,αs1 CND基因为优势基因,在麦洼牦牛和九龙牦牛中的频率分别为0.8073和0.6000,β Lg座位优势基因为β LgE,在麦洼牦牛和九龙牦牛中的频率分别为0.9770和0.9700;麦洼牦牛和九龙牦牛群体平均杂合度分别为0.1021和0.1867;在2个品种牦牛群体中,除αs1 CN基因座对九龙牦牛乳脂率有显著影响外,其余乳蛋白基因座对泌乳性能及乳蛋白相对百分含量均无显著影响。The genetic polymorphisms of milk protein were investigated by polyacrylamide gel electrophoresis (PAGE) from 109 Maiwa yak and 100 Jiulong yak, and the relationship between milk protein polymorphisms and milking traits, milk protein composition percentage were studied by least mean square (LMS). The results showed that β\|Lg, κ\|CN and α\|La were monomorphic, the genotype were AA, BB and BB respectively. αs1\|CN and β\|Lg were polymorphic, the dominant gene was αs1\|CN D, the frequency was 0.8073 and 0.6000 in Maiwa yak and Jiulong yak respectively. the dominant gene was E for β\|Lg, the frequency was 0.9770 and 0.9700 in Maiwa yak and Jiulong yak respectively. The average heterozygosity was 0.1021 and 0.1867 in Maiwa yak and Jiulong yak respectively. No significant effects were observed to milking traits and milk protein composition expect αs1\|CN locus to fat percentage in Jiulong yak.

关 键 词：麦洼牦牛 九龙牦牛 乳蛋白 遗传多态性 泌乳性状 

分 类 号：S823.85[农业科学—畜牧学]