离子对反相色谱法测定桑叶中水溶性维生素含量  

Determination of watersoluble vitamins in mulberry leaves by high performance liquid chromatography

作　　者：孙莲[1] 张丽静[2] 孟磊[1] 陈坚[3] 贾殿增[2] 

机构地区：[1]新疆医科大学基础医学院化学教研室 [2]新疆大学理化测试中心 [3]新疆医科大学药学院,新疆乌鲁木齐830000

出　　处：《新疆医科大学学报》2003年第3期263-264,267,共3页Journal of Xinjiang Medical University

摘　　要：目的 :测定新疆不同地区、不同采集期、不同品种桑叶中水溶性维生素 B1 、B2 (Vit B1 、Vit B2 )的含量 ,以此作为开发利用的标准。方法:采用离子对反相色谱法测定桑叶中的水溶性维生素含量。结果 :不同采集期桑叶中 Vit B1 和 Vit B2 的含量基本相同 ,无明显的变化规律 ,Vit B1 和 Vit B2 的平均含量分别为 6 .10、2 2 .4 μg/g,Vit B2的含量大约是 Vit B1 含量的 3倍。南疆地区 (阿克苏、喀什、和田 )的桑叶中 Vit B1 和 Vit B2 的含量比北疆 (乌鲁木齐 )和东疆 (哈密、吐鲁番 )地区桑叶中的含量略高。黑桑和白桑中 Vit B1 和 Vit B2 的含量基本相同。 Vit B1 和 Vit B2的平均回收率分别为 97.2 %和 97.5 % ,Vit B1 和 Vit B2 含量的 RSD分别为 3.4 5 % (n=6 )和 1.5 4 % (n=6 )。 结论 :用离子对反相色谱法测定桑叶中的水溶性维生素 ,方法简单、快速 ,结果准确 ,重现性好。Objectives: To determine vitamineB\-1 and vitamineB\-2 in mulberry leaves collected in different periods, different places and different kinds in Xinjiang. Methods: A HPLC method was used. Results: The contents of vitamineB\-1 and vitamineB\-2 in mulberry leaves of every month were almost equal, the average content of vitamineB\-2 was three times as high as that of vitamineB\-1. The contents of vitamineB\-1 and vitamineB\-2 in mulberry leaves of sorthen area were a little higher than that of northen area and easten area. The contents of vitamineB\-1 and vitamineB\-2 in mulberry leaves of two kinds were almost equal. The average recoveries were 97.2% for vitamineB\-1 and 97.5% for vitamineB\-2; the RSD were 3.45% (n=6) for vitamineB\-1 and 1.54% (n=6) for vitamineB\-2. Conclusion: The results suggest that this method is simple, specific and accurate.

关 键 词：离子对反相色谱法 测定 桑叶 水溶性维生素 含量 

分 类 号：R284.1[医药卫生—中药学]