食管癌患者3p25等位基因杂合缺失的初步研究  

Detection of allele-specific chromosome 3p25 by PCR -LOH in 35 cases of esophageal carcinoma

作　　者：胡燕荣[1] 吴明拜[2] 赵学信[1] 张铸[2] 武贵臻[1] 伊利亚尔.夏合丁 贺捷[1] 

机构地区：[1]新疆医科大学基础医学院生物化学教研室,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院胸外科,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2003年第3期238-240,共3页Journal of Xinjiang Medical University

摘　　要：目的 :检测 35例食管癌患者 3p2 5 D3S10 38染色体位点的杂合缺失 ,为寻找此位点附近潜在的抑癌基因奠定基础。 方法 :微卫星 DNA- PCR-银染法检测 3p2 5 D3S10 38位点杂合缺失。结果:3p2 5 D3S10 38位点有 11例存在杂合缺失 (L OH) ,L OH率为 31.4 % ,此位点杂合缺失在各民族中均存在。结论:3p2 5 D3S10 38存在杂合缺失且有一定发生频率 ,可能与食管癌的发生有关 ,值得继续研究。Objective: To study loss of heterozygosity at chromosome 3p25 in 35 patients with esophageal carcinoma. Methods: The loss of heterozygosity at 3p25 was conducted by using microsatellite DNA PCR silver staining method.Results: The rate of LOH at 3p25D3S1038 was 31.4%.Conclusion: There is alteration of microsatellite marker on chromosome 3p25, which is valuable and worthy to study further.

关 键 词：食管癌 3p25 等位基因杂合缺失 PCR—RFLP 

分 类 号：R735.1[医药卫生—肿瘤]