太平洋牡蛎人工诱导雌核发育精子遗传失活的初步研究  

Genetic inactivity of Pacific oyster sperm by ultraviolet light for gynogenesis

作　　者：李雅娟[1] 毛连菊[1] 李霞[1] 丁君[1] 赵艳荣[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁大连116023

出　　处：《大连水产学院学报》2003年第2期109-113,共5页Journal of Dalian Fisheries University

摘　　要：用紫外线照射法对太平洋牡蛎Crassostreagigas精子进行不同照射剂量和照射时间的处理,并将处理后的精子与正常的太平洋牡蛎的卵进行授精实验。实验结果表明,在精子厚度为1mm时,紫外线的最佳照射时间为1min、最佳照射剂量为1500μW/cm2,受精率为87 74%,早期胚胎成活率为92 97%,单倍体率为96 24%。实验中,在担轮幼虫期利用染色体计数和流式细胞仪测定单倍体率,并从早期胚胎存活率上观察到"Hertwig"效应的存在。The sperms of Pacific oyster (Crassostrea gigas) were irradiated by UV light with different dose and time, then the activated eggs were fertilized by these sperms. The results showed that the optimal irradiation time and dose were 1 min and 1500 μW/cm2, fertilization rate 87.74%, survial rate of early embryo stage 92.97%, haploid rate 96.24% when sperms thickness was 1 mm. The haploid rate was determined by means of counting chromosome of trochophores and Flow cytometry, and there was the 'Hertwig' effect observed from the survival rate of early embryo.

关 键 词：太平洋牡蛎 雌核发育 人工诱导 精子遗传失活 紫外线照射法 照射剂量 照射时间 受精率 早期胚胎成活率 单倍体率 

分 类 号：S968.311[农业科学—水产养殖]