微生态制剂对肝内胆汁淤积性肝损伤兔模型胆汁和血清TNF-a、IL-6的影响  

THE EFFECTS OF MICROECOLOGICAL PREPARATION IN THE BILE AND SERUM TNF-A、IL-6 OF THE RABBIT-MODELS OF INTRAHEPATIC CHOLESTATIC HEPATIC INJURY

作　　者：黄欣[1] 黄志华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《中国微生态学杂志》2003年第3期137-140,共4页Chinese Journal of Microecology

摘　　要：目的 :通过研究异硫氰酸萘酯 (ANIT) )所致的急性肝内胆汁淤积性肝损伤兔模型血清和胆汁中TNF a、IL 6的代谢变化 ,来进一步探讨微生态制剂 乐托尔对该模型的作用机制。方法 :按 2 0 0mg/kg一次性灌服ANIT来诱发新生白兔急性肝内胆汁淤积性肝损伤病变 ,分别检测中毒后 2 4、4 8h及用乐托尔散剂治疗后血清和胆汁中总胆红素 (TB)、丙氨酸转氨酶 (ALT)、肿瘤坏死因子 a(TNF a)、白细胞介素 6(IL 6 )的值。结果 :在乐托尔组中发现 ,灌服ANIT 4 8h后血清和胆汁中的TNF a、IL 6、ALT、TB值均较中毒 4 8h组明显降低 (P值均小于 0 0 5 ) ;各组胆汁中TNF a、IL 6的浓度均明显高于血清中浓度。结论 :微生态制剂能够改善肝功能 ,增加胆汁的排泄量 ,使炎性细胞因子的产生量显著减少。Objective:To investigate the metabolic alteration of the bile and serum TNF-a?IL-6 in the rabbit-models of acute intrahepatic cholestatic hepatic injury induced by alpha-haphtylisocyocyanate(ANIT),and further discuss the mechanism of the microecological preparation-Lacteol Fort to this models. Methods: The intrahepatic cholestatic hepatic injury models was induced by a single administration (200 mg/kg) of alpha-naphthyliso-thiocyanate(ANIT),and detected the levels of the serum and bile total bilirubin(TB)?alanine aminotransferase (ALT)?tumor necrosis factor-a(TNF-a)?interleukin-6(IL-6) in the intoxication groups of 24 h and 48 h after the administration and the Lactéol Fort-treated group,after the administration of ANIT,the levels of bile and serum TNF-a?IL-6?ALT?TB?all decreased distinctly than the intoxication groups of the serum. Conclusion: The mivroecological bile fluid and decrease the production of the proinflammatory cytokines distinctly.

关 键 词：微生态制剂 肝内胆汁淤积 肝损伤 兔模型 胆汁 血清 TNF-α IL-6 细胞因子 

分 类 号：R575.22[医药卫生—消化系统]