二氯五氟丙烷的28天吸入毒性试验观察  

Observation on 28-day Inhalation toxicity of HCFC-225

作　　者：董竞武[1] 肖萍[1] 潘喜华[1] 郑勇英[1] 叶秀玲[1] 顾文华[1] 符振华[1] 仲伟鉴[1] 

机构地区：[1]上海市疾病预防控制中心毒理室,上海200336

出　　处：《环境与职业医学》2003年第3期236-238,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的 ]研究二氯五氟丙烷 (HCFC 2 2 5 )的毒性及安全性。 [方法 ]通过实验动物急性毒性试验来确定该物质的急性毒性等级 ,通过大鼠 2 8天吸入毒性试验找出亚急性毒作用浓度阈值及靶器官。急性毒性用霍恩氏法计算 ;亚急性毒性用不同剂量的该物质对动物染毒 ,分别对各剂量组的动物进行血液生化测定 ,称量动物体重、脏器重量并进行组织病理学检查 (肺、肝、肾、心 )。 [结果 ]小鼠急性LD50 雌性为 2 0g/kg ,雄性为 14 7g/kg ;小鼠急性LC50 为 5 2 6670mg/m3 ;大鼠急性LD50 大于 2 1 5g/kg ;大鼠 2 8d吸入毒性试验结果提示 ,AK 2 2 5吸入毒作用的主要靶器官为动物的肺脏和肝脏 ,其次是肾脏。主要的毒性表现有血清中ALT、AST、CR、BUN含量升高 ;肺组织淤血、出血及炎症细胞浸润 ;肝组织轻度及水样变病理改变等。最小毒作用浓度 (LOEC)为 3 73 5 0mg/m3 ,未观察到毒作用浓度 (NOEC)为 12 45 0mg/m3 。 [结论 ]二氯五氟丙烷 (HCFC 2 2 5 )的急性毒性属于微毒 ;本次大鼠 2 8d吸入毒性试验结果提示 ,HCFC 2 2 5吸入毒作用的主要靶器官为动物的肺脏和肝脏 ,其次是肾脏。最小毒作用浓度 (LOEC)为 3 73 5 0mg/m3 ,未观察到毒作用浓度 (NOEC)为 12 45 0mg/m3 。Objective] To study the toxicity of HCFC 225 and its security. [Methods] To confirm acute toxicity grade by acute toxicity test in experiment animal. To find out the target organ and the concentrations for testing subacute toxicity by a 28 day repeated inhalation exposure. [Results] Acute peroral LD 50 for female mice is 20 g/kg,for male mice is 14 7 g/kg.Acute inhalation LC 50 for mice is 526 670 mg/m 3.Acute peroral LD 50 for rats is more than 21 5 g/kg. The result of day 28 repeated inhalation study in rats showed that the main target organs after inhalation of HCFC 225 were lung,liver and then kidney. Toxicity was shown that the serum ALT,AST,CR and BUN increased;gore,bleeding and infiltration of phlogostic cells;and pathologic changes such as light cloudy swelling and aquosity. The Lowest Observed Effect Concentration(LOEC) is 37 350 mg/m 3. The No Observed Effect Concentration (NOEC) is 12 450 mg/m 3. [Conclusion] HCFC 225 belongs to slight toxicity grade. The result of 28 day repeated inhalation study in rats shows the main target organs after inhalation of HCFC 225 are lung,liver and then kidney. LOEC is 37 350 mg/m 3. NOEC is 12 450 mg/m 3.

关 键 词：二氯五氟丙烷 28天吸入毒性试验 安全性 大鼠 剂量 毒理学 

分 类 号：R994.3[医药卫生—毒理学]