代谢工程技术调控3-羟基丁酸与3-羟基己酸共聚酯PHBHHx的微生物合成  

Metabolic engineering for microbial production of copolyesters consisting of 3 - hydroxybutyrate and 3 - hydroxyhexanoate with adjustable 3 - hydroxyhexanoate content

作　　者：欧阳少平[1] 丘远征[1] 卢晓云[1] 吴琼[1] 陈国强[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084

出　　处：《生物加工过程》2003年第1期60-65,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(30170017;20074020号)资助项目

摘　　要：3-羟基丁酸和3-羟基己酸共聚酯(PHBHHx)是一种新型生物可降解材料,其性能与3-羟基己酸(3HHx)在共聚物中的摩尔百分含量密切相关。本研究在两株嗜水性气单孢菌Aeromonas WQ和Aeromonas hy-drophila 4AK4中分别引入了编码酯酰辅酶A脱氢酶的yafH基因和编码合成3-羟基丁酸-CoA的phbA和phbB基因,将A.hydrophila WQ合成的PHBHHx中的3HHx的摩尔含量由3％-5％提高到20％以上;而A.hydrophila 4AK4合成的PHBHHx中的3HHx摩尔含量则由15％左右降低到3％～12％。成功地实现了对PHBHHx单体组成的调控。Copolyesters consisting of 3 - hydroxybutyrate (3HB) and 3 - hydroxyhexanoate (3HHx) (PHBH-Hx) were synthesized by two recombinant Aeromonas hydrophila strains. The introduction of yafH gene into A . hydrophila WQ led to an increase of 3HHx fraction in PHBHHx from 3 % - 5 % in the wild type strain to over 20% in the recombinant, while foreign genes of phbA and phbB genes in A. hydrophila 4AK4 decreased 3HHx fraction from 15% in the wild type to a desired level of 3% - 12% in the recombinant. Gluconate can also be used to control the 3HHx content in the copolyesters.

关 键 词：代谢工程技术 调控 3-羟基丁酸与3-羟基己酸共聚酯 PHBHHX 微生物合成 嗜水性气单孢菌 

分 类 号：TQ929[轻工技术与工程—发酵工程] Q936[生物学—微生物学]