视网膜微血管内皮细胞离子通道的特性研究  

Study on the Ion Channel Character of the Bovine Retinal Microvascular Endothelial Cells

作　　者：邱萍[1] 王士雯[1] 温宁[2] 张秀阁 张作明[4] 

机构地区：[1]解放军总医院老年心血管病研究所,北京100853 [2]解放军总医院口腔科 [3]总后第一门诊部 [4]第四军医大学空军医学系航空临床教研室

出　　处：《中国微循环》2003年第3期163-166,共4页Journal of Chinese Microcirculation

基　　金：国家自然科学基金(No.39900162);军队九五重点课题资助(No.96Z042)

摘　　要：目的 探讨视网膜微血管内皮细胞离子通道特性。方法 膜片钳技术的全细胞记录方式。结果 原代培养牛视网膜微血管内皮细胞的静息膜电位为(-34.711±2.196)mV(n=46),几乎每次记录都发现相似的跨膜外向电流,该电流具有电压依赖性及不失活特性,能被已知的Ca^(2+)激活K^+通道特异性阻抑剂不同程度阻断。结论 在视网膜微血管内皮细胞上存在Ca^(2+)激活K^+通道。Objective To investigate ion channel character of the bovine retinal microvessels endothelial cells (BREC). Methods Whole-cell recording mode was used to research the membrane potential and membrane current of BREC. Results The special K+ channel blockers were used to identify the types of K+ channels. The resting potential of BREC is - 34.711 ± 2.196mV (n = 46) . The records of transmemebrane current from most cells were similar. The current was voltage-dependent and could be blocked by the known Ca2+ -activated-k+ channel blocker. Conclusions The transmembrane current is composed mostly of BKCa in retinal microvascular endothelium.

关 键 词：膜片钳技术 微血管内皮细胞 Ca^2+激活K^+通道 离子通道 视网膜 

分 类 号：R774.1[医药卫生—眼科]