单粒活体稻谷种子直链淀粉含量的近红外透射光谱分析  

Determination of Amylose Content in Single Rice Grain by Near Infrared Transmittance Spectroscopy(NITS)

作　　者：肖昕[1] 陈奕[1] 罗文永[1] 刘彦卓[1] 毛兴学[1] 李晓方[1] 

机构地区：[1]广东省农业科学院水稻研究所,广东省水稻育种新技术重点实验室,广东广州510640

出　　处：《中国水稻科学》2003年第3期287-290,共4页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目 ;广东省自然科学基金资助项目 ;广东省科技计划资助项目 ;广东省农业厅"人大农业科学技术推广议案"资助项目 ;农业部"948"资助项目 ;广州市科技计划资助项目

摘　　要：利用FOSSTecator公司的Infratec 12 5 5型带单粒定标器的近红外谷物分析仪 ,对 2 2 2粒单粒稻谷进行扫描并测定了直链淀粉含量的参比数据。借助于功能强大的近红外定标器软件 (WinISI) ,采用多种计量数学处理方法和不同的回归统计方法进行定标曲线的开发和比较 ,优化得到了单粒水稻种子直链淀粉含量测定的近红外定标方程。其定标标准偏差(SEC)、交叉检验标准误差 (SECV)、检验工作标准误差 (SEP)和定标相关系数 (RSQ)分别为 2 .82 8、3.0 88、2 .792、0 .848。Two hundred and twenty-two samples of single rice seed were scanned by FOSS Tecator Infratec 1255 Analyzer with Single Seed Adapter and the amylose contents were determined by reference method. With the powerful WinISI calibration maker software, several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of amylose content in a single rice grain. The standard error of calibration (SEC), standard error of cross-validation(SECV), validation performance (SEP) and regression squared (RSQ) of the calibration selected were 2.828, 3.088, 2.792 and 0.848, respectively.

关 键 词：直链淀粉 含量 近红外透射光谱分析 水稻 单粒种子 

分 类 号：O657.3[理学—分析化学]