气相色谱法测定水产品中氯霉素残留  

Determination of chloramphenicol residues in aquatic products by gas chromatography

作　　者：赵文亚[1] 沈美芳 徐幸莲[1] 吴光红 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]江苏省水产质量检测中心,江苏南京210017

出　　处：《水产学报》2003年第3期278-282,共5页Journal of Fisheries of China

基　　金：江苏省"十五"科技攻关资助项目(BE2001385)

摘　　要：介绍了水产品中氯霉素残留量测定的气相色谱法。样品中的氯霉素用乙酸乙酯提取,正己烷去脂肪,过Sep-C18柱进行净化,用BSTFA-TMCS衍生后进带有微电子俘获器的气相色谱仪检测。该方法的线性范围为0.5～500μg·L-1,相关系数r=0.999,最低检测限为0.1μg·kg-1,相对标准偏差为4.3%～11.0%,向样品中分别添加1μg·kg-1、10μg·kg-1和20μg·kg-13个浓度水平的氯霉素,回收率分别为62.0%、88.2%和96.4%。方法灵敏度高,准确可靠,适合水产品中氯霉素残留量的检测。A gas chromatography method had been established for the determination of chloramphenicol in aquatic products. Chloramphenicol was extracted with ethyl acetate and partitioned with nhexane to remove lipids. Cleanup was performed on a Sep cartridge. After the drug was derived with BSTFA-TMCS,the derivatives were analyzed by capillary gas chromatography-electron capture detection. The linear range was from 0.5μg·L-1 to 500μg·L-1 with r of 0.999. The average recoveries were 62.0%, 88.2% and 96.4% respectively when samples were spiked with 1μg·kg-1, 10μg·kg-1 and 20μg·kg-1. The detection limit was 0.1μg·kg-1. The RSDs were 4.3%-11.0%. The method is sensitive, accurate and suitable for the determination of trace chloramphenicol in aquatic products.

关 键 词：气相色谱法 测定技术 水产品 氯霉素 残留量 氯霉素 乙酸乙酯 微电子俘获器 

分 类 号：X592[环境科学与工程—环境工程]